Life Sciences

Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes

Abstract

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®– Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

Molecular Analysis of Biofield Treated Eggplant and Watermelon Crops

Abstract

Eggplant and watermelon, as one of the important vegetative crops have grown worldwide. The aim of the present study was to analyze the overall growth of the two inbreed crops varieties after the biofield energy treatment. The plots were selected for the study, and divided into two parts, control and treated. The control plots were left as untreated, while the treated plots were exposed with Mr. Trivedi’s biofield energy treatment. Both the crops were cultivated in different fields and were analyzed for the growth contributing parameters as compared with their respective control. To study the genetic variability in both plants after biofield energy treatment, DNA fingerprinting was performed using RAPD method. The eggplants were reported to have uniform colored, glossy, and greener leaves, which are bigger in size. The canopy of the eggplant was larger with early fruiting, while the fruits have uniform shape and the texture as compared with the control. However, the watermelon plants after the biofield treatment showed higher survival rate, with larger canopy, bright and dark green leaves compared with the untreated plants. The percentage of true polymorphism observed between control and treated samples of eggplant and watermelon seed samples were an average value of 18% and 17%, respectively. Overall, the data suggest that Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate, and can be utilized in better way as compared with the existing agricultural crop improvement techniques to improve the overall crop yield.

Evaluation of Plant Growth, Yield and Yield Attributes of Biofield Energy Treated Mustard (Brassica juncea) and Chick Pea (Cicer arietinum) Seeds

Abstract

The present study was carried out to evaluate the effect of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) and chick pea (Cicer arietinum) for their growth, yield, and yield attributes. Both the samples were divided into two groups. One group was remained as untreated and coded as control, while the other group (both seed and plot) was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated. The result showed the plant height of mustard and chick pea was increased by 13.2 and 97.41%, respectively in the treated samples as compared to the control. Additionally, primary branching of mustard and chick pea was improved by 7.4 and 19.84%, respectively in the treated sample as compared to the control. The control mustard and chick pea crops showed high rate of infection by pests and diseases, while treated crops were free from any infection of pests and disease. The yield attributing characters of mustard showed, lucidly higher numbers of siliquae on main shoot, siliquae/plant and siliquae length were observed in the treated seeds and plot as compared with the control. Moreover, similar results were observed in the yield attributing parameters of chick pea viz. pods/plant, grains/pod as well as test weight of 1000 grains. The seed and stover yield of mustard in treated plots were increased by 61.5% and 25.4%, respectively with respect to the control. However, grain/seed yield of mustard crop after biofield energy treatment was increased by 500% in terms of kg per meter square as compared to the control. Besides, grain/seed yield of chick pea crop after biofield energy treatment was increased by 500% in terms of kg per meter square. The harvest index of biofield treated mustard was increased by 21.83%, while it was slight increased in case of chick pea. In conclusion, the biofield energy treatment could be used on both the seeds and plots of mustard and chick pea as an alternative way to increase the production and yield.

Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media

Abstract

The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (Tonset) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, Tmax (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165→3130 cm-1 (aromatic C-H stretching); 2813→2775 cm-1 (aliphatic C-H stretching); 1145→1137 cm-1 (C-N stretching), 995→1001 cm-1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (λmax) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.

Assessment of Antibiogram of Biofield Energy Treated Serratia marcescens

Abstract

Serratia marcescens (S. marcescens) has become an important nosocomial pathogens and increased resistant isolates were reported. The current study evaluates the impact of an alternate energy medicine i.e. Mr. Trivedi’s biofield energy treatment on S. marcescens for changes in sensitivity pattern of antimicrobial, biochemical characteristics, and biotype number. S. marcescens cells were procured from MicroBioLogics Inc., USA in sealed pack bearing the American Type Culture Collection (ATCC 13880) number and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15 respectively. All experimental parameters were studied using the automated MicroScan Walk-Away® system. Antimicrobial susceptibility results showed that 42.85% of tested antimicrobials results in altered sensitivity pattern, while decreased minimum inhibitory concentration values in 40.62% tested antimicrobials as compared to the control after biofield treatment on S. marcescens. The biochemical study showed that 12 out of 33 tested biochemicals (36.36%) were reported for alteration of biochemical reactions pattern as compared to the control. Biotype study showed an alteration in biotype number in all the experimental treated groups as compared to the control. These results suggested that biofield energy treatment has a significant impact on S. marcescens. Overall, it is expected that Mr. Trivedi’s biofield energy treatment as an integrative medicine could be better therapy approach in near future.

Morphological Characterization, Quality, Yield and DNA Fingerprinting of Biofield Energy Treated Alphonso Mango (Mangifera indica L.)

Abstract

Alphonso is the most delicious variety of mango (Mangifera indica L.) known for its excellent texture, taste, and richness with vitamins and minerals. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on morphological characteristics, quality, yield and molecular assessment of mango. A plot of 16 acres lands used for this study with already grown mango trees. This plot was divided into two parts. One part was considered as control, while another part was subjected to Mr. Trivedi’s biofield energy treatment without physically touching and referred as treated. The treated mango trees showed new straight leaves, without any distortion and infection, whereas the control trees showed very few, distorted, infected, and curly leaves. Moreover, the flowering pattern of control trees did not alter; it was on average 8 to 10 inches with more male flowers. However, the flowering pattern of treated trees was completely transformed into compact one being 4 to 5 inches in length and having more female flowers. Additionally, the weight of matured ripened mango was found on an average 275 gm, medium sized with 50% lesser pulp in the control fruits, while the fruits of biofield energy treated trees showed on average weight of 400 gm, large sized and having 75% higher pulp as compared to the control. Apart from morphology, the quality and nutritional components of mango fruits such as acidity content was increased by 65.63% in the treated sample. Vitamin C content in the treated Alphonso mango pulp was 43.75% higher than the pulp obtained from the control mango farm. The spongy tissue content in pulp of the matured ripened mangoes was decreased by 100% for two consecutive years as compared to the control. Moreover, the yield of flowers and fruits in the treated trees were increased about 95.45 and 47.37%, respectively as compared to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample did not show any true polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on the mango trees showed a significant improvement in the morphology, quality and overall productivity along with 100% reduction in the spongy tissue disorder. In conclusion, the biofield energy treatment could be used as an alternative way to increase the production of quality mangoes.

Impact of Biofield Energy Treatment on Soil Fertility

Abstract

Measurement of soil components such as microbial population, minerals and obviously the content of organic carbon play the important roles for the productivity of crops and plants. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on soil for its physical (electrical conductivity), chemical (minerals) and microbial flora (bacteria and fungi). A plot of lands was assigned for this study with some already grown plants. This plot was divided into two parts. One part was considered as control, while another part was subjected to Mr. Trivedi’s biofield energy treatment without physically touching and referred as treated. In the treated soil the total bacterial and fungal counts were increased by 546 and 617%, respectively as compared to the untreated soil. Additionally, the conductivity of soil of the treated plot was increased by 79% as compared to the soil of control plot. Apart from microbes, the content of various minerals were also changed in the biofield energy treated soil. The calcium carbonate content showed 2909 ppm in the control, while in the treated soil it was increased to 3943 ppm i.e. 36% increased. Various other minerals such as nitrogen and potassium were increased by 12% and 7%, respectively as compared to the control. Besides, the level of some minerals such as potassium, iron, and chloride were decreased by 9%, 23%, and 41%, respectively as compared to the control. Apart from chemical constituents of soil, the content of organic carbon was also reduced by 8% in the treated soil as compared to the control soil. The overall results envisaged that the biofield energy treatment on the soil showed a significant improvement in the physical, chemical, and microbial functions of soil component. Thus, improved the conductance, supportive microbes, minerals and overall productivity of crops. In conclusion, the biofield energy treatment could be used as an alternative way to increase the yield of quality crops by increasing soil fertility.

Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone

Abstract

Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.

Physical, Spectroscopic and Thermal Characterization of Biofield Treated Fish Peptone

Abstract

The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Trivedi’s biofield treatment, coded as the treated sample and another part was coded as the control. The impact of biofield treatment was analysed through various analytical techniques and results were compared with the control sample. The particle size data revealed 4.61% increase in the average particle size (d50) along with 2.66% reduction in the surface area of the treated sample as compared to the control. The X-ray diffraction studies revealed the amorphous nature of the fish peptone sample; however no alteration was found in the diffractogram of the treated sample with respect to the control. The Fourier transform infrared studies showed the alterations in the frequency of peaks corresponding to N-H, C-H, C=O, C-N, and C-OH, functional groups in the treated sample as compared to the control. The differential scanning calorimetry data revealed the increase in transition enthalpy (ΔH) from -71.14 J/g (control) to -105.32 J/g in the treated sample. The thermal gravimetric analysis data showed the increase in maximum thermal degradation temperature (Tmax) from 213.31°C (control) to 221.38°C along with a reduction in the percent weight loss of the treated sample during the thermal degradation event. These data revealed the increase in thermal stability of the treated fish peptone and suggested that the biofield energy treatment may be used to improve the thermal stability of the heat sensitive compounds.

Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium

Abstract

Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS) has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’s i.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment. The control and treated samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis indicated the increase in crystallite size by 5.20% in treated PBS as compared to the control. Similarly, the treated HBS also showed increase in crystallite size by 3.20% with respect to the control. Additionally, the treated PBS showed an increase in Bragg’s angle (2θ) as compared to the control sample. However, a decrease in Bragg’s angle of XRD peaks of the treated sample was noticed in the treated HBS. The DSC analysis of the control PBS showed melting temperature at 224.84°C; however melting temperature was not observed in the treated sample. However, DSC analysis of the treated HBS showed an increase in melting temperature (152.83°C) in comparison with the control (150.60°C). Additionally, the latent heat of fusion of the treated HBS was increased substantially by 108.83% as compared to the control. The TGA thermogram of the treated PBS showed an increase in onset of thermal degradation (212°C) as compared to the control (199°C). Whereas, the treated HBS showed less weight loss comparing with the control sample. This indicated the increase in thermal stability of the both the treated PBS and HBS samples. The FT-IR spectroscopic analysis of treated PBS showed alterations in the frequency of the functional groups such as O-H, C-H, P=O, O=P-OH, and P-OH as compared to the control. Additionally, the FT-IR spectrum of the treated HBS showed increase in frequency of calcium chloride phase (1444→1448 cm-1) as compared to the control sample. Altogether, it was observed that biofield energy treatment had caused physical, thermal and spectral changes in the treated samples as compared to the control. It is assumed that biofield energy treated PBS and HBS could be a good prospect for biological and tissue culture applications.

Physicochemical and Spectroscopic Characterization of Yeast Extract Powder After the Biofield Energy Treatment

Abstract

Yeast extract powder (YE powder) is particularly used in culture media for the cultivation of microorganisms found in milk or other dairy products. The present study was intended to explore the influence of biofield energy treatment on the physicochemical and spectral properties of YE powder. The study was accomplished in two groups; first group was remained as control, while another was subjected to Mr. Trivedi’s biofield energy treatment and termed as the treated group. Afterward, both the samples were evaluated using several analytical techniques. The X-ray diffractometry (XRD) study showed the halo patterns of XRD peaks in both the samples. This indicated the amorphous nature of the samples. The particle size study revealed the 4.77% and 26.28% increase d50 (in the average particle size) and d99 (particle size below that 99% particles are present), respectively of treated YE powder with respect to the control. The surface area analysis showed the 14.06% increase in the specific surface area of treated sample with respect to the control. The differential scanning calorimetry (DSC) analysis exhibited the 41.64% increase in the melting temperature of treated YE powder sample as compared to the control. The TGA/DTG analysis exhibited the increase in Tonset (onset temperature of thermal degradation) by 7.51% and 12.45% in first and second step of thermal degradation, respectively in the treated sample as compared to the control. Furthermore, the Tmax (maximum thermal degradation temperature) was increased by 4.16% and 24.79% in first and second step of thermal degradation, respectively in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as C-H (stretching) from 2895→2883 cm-1 and 2815→2831 cm-1, respectively; C-N from 1230→1242 cm-1; and C-O stretching from 1062-1147 cm-1→1072-1149 cm-1 of treated YE powder sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) in both the control and treated samples. Therefore, the analytical results suggested the considerable impact of Mr. Trivedi’s biofield energy treatment on physicochemical and spectral properties of YE powder. The increase in Tonset and Tmax after the biofield treatment suggests that the treated YE powder might be more effective in culture medium than the control YE powder.

Physicochemical Evaluation of Biofield Treated Peptone And Malmgren Modified Terrestrial Orchid Medium

Abstract

Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer and surface area analyzer. The XRD analysis revealed the amorphous nature of the control and treated peptone samples. The DSC analysis showed an increase in thermal denaturation temperature of the treated peptone (196.22°C) as compared to the control sample (141.20°C). Additionally, the exothermic peak of treated sample (280°C) was increased as compared to the control (270°C). The DSC of control and treated MMTO showed the absence of the melting temperature in their respective DSC thermograms. The TGA analysis of the treated peptone showed an increase in onset of thermal degradation (172°C) with respect to the control (170°C). Nevertheless, the TGA thermogram of the treated MMTO (293.96°C) showed an increase in maximum thermal degradation temperature (Tmax) as compared with the control (281.41°C). It indicated the good thermal stability of the treated peptone and MMTO samples. The FT-IR result of the treated peptone showed an upward shift in C-H (2817→2833 cm-1), and amide I (1635→1641 cm-1), stretching in the treated sample with respect to the control sample. Whereas, the FT-IR spectrum of the treated MMTO showed an increase in the frequency of the C-H (2817→2833 cm-1) and amide I (1596→1606 cm-1) bands as compared to the control. Particle size analysis of the treated peptone showed an increase in d50 (average particle size) and d99 (size exhibited by 99% of particles) by 9.3 and 41.4% , respectively with respect to the control. Surface area analysis showed increase in surface area by 4.3% in the treated peptone. Altogether, the results corroborated that the biofield energy treatment had altered the physical, thermal and spectral properties of peptone and MMTO. It is assumed that biofield treated peptone and MMTO could be utilized as potential candidates for cell culture applications.

Evaluation of Vegetative Growth Parameters in Biofield Treated Bottle Gourd (Lagenaria siceraria) and Okra (Abelmoschus esculentus)

Abstract

The objective of the study was to assess the growth contributing characters of biofield treated bottle gourd (Lagenaria siceraria) and okra (Abelmoschus esculentus) seeds. The seeds of both crops were divided into two groups, one was kept aside and denoted as untreated, while the other group was subjected biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. Further the level of glutathione (GSH) in okra leaves, along with DNA fingerprinting in bottle gourd were analyzed using RAPD method. After germination, the plants of bottle gourd were reported to be strong and erect with better canopy as compared with the control. The vegetative growth of okra plants after biofield energy treatment was found to be stout with small canopy, strong steam, and more fruits per nodes, that contributed high yield as compared with the control. However, endogenous level of GSH in the leaves of okra was increased by 47.65% as compared to the untreated group, which may suggest an improved immunity of okra crops. Besides, the DNA fingerprinting data, showed polymorphism (42% ) between treated and untreated samples of bottle gourd. The overall results suggest that the biofield energy treatment on bottle gourd and okra seeds, results an improved overall growth of plant and yield, which may enhance flowering and fruiting per plant. Study results conclude that the biofield energy treatment could be an alternate method to improve the crop yield in agricultural science.

Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd

Abstract

Plants are known to have sense and can respond to touch, electric and magnetic field. The present study was designed on the sponge gourd (Luffa cylindrica) and bitter gourd (Momordica charantia) seeds with respect to biofield energy treatment. The seeds of each crop were divided into two groups, one was kept control, while the other group was subjected to Mr. Trivedi’ biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. To study the genetic variability after biofield energy treatment, both the seeds were analyzed for DNA fingerprinting using RAPD method. After germination, the plants of sponge gourd were reported to have uniform colored leaves and strong stem. The leaves and fruits of sponge gourd showed no infection, with anti-gravity properties during early stage of fruiting as compared with the control group. Similarly, treated bitter gourd showed uniform color of leaves, strong stem, with disease free fruits in biofield treated seeds as compared with the control. The true polymorphism (% ) observed between control and treated samples of sponge and bottle gourd seed sample was an average value of 7.8% and 66% respectively. In conclusion, Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate that may by interacting with plant genome, which resulted in high yield of crops.

Effect of Biofield Energy Treatment on Chlorophyll Content, Pathological Study, and Molecular Analysis of Cashew Plant (Anacardium occidentale L.)

Abstract

In the world scenario, India occupies a premier position contributing to about 43 per cent production of the cashew nut (Anacardium occidentale L.) along with export and processing. The aim is to study the impact of biofield energy treatment on selected farms for cashew farming. The control and biofield treated farms were divided as control and treated farms, and Mr. Trivedi provided the biofield energy treatment to the treated farms. Further, the plants and fruits were analyzed for overall growth of plants, chlorophyll content, productivity, pathological study, and shelf life using UN specifications for International Trade, biophoton emission study, and DNA fingerprinting using RAPD method. No chemicals, fertilizers, were used on the treated plot, although regular practices were followed on control farms such as fertilizers, pesticides and fungicides due to the high incidence of disease and the requirement of nutritional supplements in the region. The analysis showed that biofield treated farm plants have thicker and stronger branches with more secondary and tertiary branches, flowering pattern, and canopy of plants was improved than trees of the same variety along with height of the plants, as compared with the control. The results showed that chlorophyll a and b content in biofield treated lands plants were increased by approximately 30% and 93% respectively, while total chlorophyll content by 45% as compared with the control. The pathological examination showed the presence of fungi namely Colletotrichum gloeosporioides and Botryodiplodia theobromae in control, which were absent in treated plants. Biophoton study suggested that the cashew fruits were bigger in size with high density, strength, and vitality as compared with the control. The shelf life analysis reflected that the biofield treated cashews showed sweet taste, and can be stored for longer duration due to less moisture, and altered minerals content, such as high iodine, and low p-anisidine level. RAPD analysis showed a high level of polymorphism among control and treated samples, while level of true polymorphism among V4 variety of cashew was ranges from 0 to 100% , and in V7 variety, it ranged from 25 to 91% using different set of RAPD primers. Overall, study results suggest that Mr. Trivedi’s biofield energy treatment on land planted with cashew could be an alternative approach to improve the overall growth of plant, and fruit yield.

Physicochemical Characterization of Biofield Treated Orchid Maintenance/Replate Medium

Abstract

Orchids are used worldwide for indoor decoration, vanilla production, and beverage preparation. They are also reported for their therapeutic efficacy in brain-related problems. The in vitro micropropagation technique was used for their propagation using the orchid maintenance/replate (OMR) medium. The current study was based on analysing the effect of biofield energy treatment on the physicochemical properties of OMR medium. A part of the sample was treated with Mr. Trivedi’s biofield energy; various physicochemical properties were analyzed and compared with the untreated (control) part. The X-ray diffraction analysis revealed the decrease in crystallite size of treated sample (132.80 nm) as compared to the control (147.55 nm). The particle size analysis revealed 20.78% increase in average particle size and 39.29% increase in d99 (size below which 99% particles are present) of the treated OMR medium as compared to the control. Moreover, the surface area of the treated sample was reduced by 3.9%, supporting the data of particle size analysis. The thermal analysis studies revealed an increase in the thermal stability of the treated OMR medium as compared to the control. The analysis was done by using differential scanning calorimetry that showed increase in melting point (1.23%) and latent heat of fusion (135.7%); and thermogravimetric analysis that reported increase in onset temperature and maximum thermal degradation temperature of the treated sample as compared to the control. Besides, the CHNSO analysis revealed the increase in percentage of nitrogen (22.22%) as well as the presence of sulphur in the treated sample. The Fourier transform infrared and UV-visible spectroscopy also showed the differences in the spectra of the treated sample as compared to the control OMR medium. Hence, the overall data revealed the impact of biofield energy treatment on the physicochemical properties of the treated sample that might be used in better way in the in vitro culture techniques as compared to the control sample.

Evaluation of Biochemical Marker – Glutathione and DNA Fingerprinting of Biofield Energy Treated Oryza sativa

Abstract

Food production needs to increase to satisfy the demand due to increasing human population worldwide. To minimize this food crisis, an increase in the rice production is necessary in many countries. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on rice (Oryza sativa) for its growth-germination of seedling, glutathione (GSH) content in seedling and mature plants, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of O. sativa cv, 644 was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi for biofield energy treatment and denoted as treated sample. The growth-germination of O. sativa seedling data exhibited that the biofield treated seeds was germinated faster on day 3 as compared to control (on day 5). The shoot and root length of seedling was slightly increased in the treated seeds of 10 days old with respect to untreated seeds. Moreover, the plant antioxidant i.e. GSH content in seedling and in mature plants was significantly increased by 639.26% and 56.24%, respectively as compared to untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was significantly (p<0.05) increased by 106.90% and 20.35%, respectively with respect to control. Besides, the DNA fingerprinting data using RAPD, revealed that the treated sample showed an average range of 5 to 46% of DNA polymorphism as compared to control. The overall results envisaged that the biofield energy treatment on rice seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the treatment of biofield energy on rice seeds could be used as an alternative way to increase the production of rice.

Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Potato Micropropagation Medium

Abstract

The micropropagation technique is used for Gerbera species due to their high demand all over the world as the decorative potted plants and cut flowers. The present study was done to investigate the impact of biofield energy treatment on the physicochemical properties of gerbera multiplication medium. A part of the sample was treated with Mr. Trivedi’s biofield energy, and the other part was kept as untreated and termed as the control sample. Both the parts were subsequently analysed for their physical, thermal and spectral properties using X-ray diffraction (XRD), particle size analysis, surface area analysis, thermogravimetric analysis (TGA), elemental analysis, and Fourier transform infrared (FT-IR) spectroscopy. The XRD results showed 13.98% increase in crystallite size of treated sample (104.01 nm) as compared to the control (91.25 nm). The particle size data revealed an increase in d50 (average particle size) and d99 (size below which 99% particles are present) by 72.57% and 42.26%, respectively of the treated sample as compared to the control. Moreover, the surface area of the treated sample was reduced from 0.694 m2/g (control) to 0.560 m2/g in the treated sample. The TGA data showed the increase in onset temperature along with the reduction in the percent weight loss of the treated sample as compared to the control. Besides, the elemental analysis revealed the significant decrease in the percentage of nitrogen (10.47%) and hydrogen (9.35%) as well as the presence of sulphur in the treated sample. The FT-IR results showed the differences in the IR frequencies corresponding to pyridine ring and N-H2 deformation of the treated sample as compared to the control. Hence, the overall data revealed that the biofield energy treatment had a significant impact on the physicochemical properties of the treated sample that might help to improve its uses in the in vitro tissue culture techniques as compared to the control sample.

Physicochemical and Spectroscopic Characterization of Biofield Energy Treated Gerbera Multiplication Medium

Abstract

Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis- differential thermal analysis (TGA-DTA), UV-Vis spectrometry, and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis showed the crystalline nature of both control and treated samples of PMM. The X-ray diffractogram showed the significant increase in the intensity of XRD peaks in treated sample as compared to the control. The XRD analysis revealed 6.64% increase in the average crystallite size of treated PMM with respect to the control. The DSC analysis showed about 8.66% decrease in the latent heat of fusion in treated sample with respect to the control. The TGA-DTA analysis exhibited about 4.71% increase in onset temperature of thermal degradation after biofield treatment with respect to the control, while the maximum thermal degradation temperature (Tmax) was also increased (5.06%) in treated sample with respect to the control. This increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. The UV spectroscopic study showed the slight blue shift in λmax of treated sample with respect to the control. FT-IR spectrum of control PMM showed the peak at 3132 cm-1 (C-H stretching) that was observed at higher wavenumber i.e. at 3161 cm-1 in the treated sample. Other vibrational peaks in the treated sample were observed in the similar region as that of the control. Altogether, the XRD, DSC, TGA-DTA, UV-Vis, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physicochemical properties of PMM. This treated PMM might be more effective as a micropropagation medium as compared to the control.

Characterization of Physical, Thermal and Spectral Properties of Biofield Treated Date Palm Callus Initiation Medium

Abstract

The date palm is mainly cultivated for the production of sweet fruit. Date palm callus initiation medium (DPCIM) is used for plant tissue culture applications. The present work is intended to evaluate the impact of Mr. Trivedi’s biofield energy treatment on physical, thermal and spectral properties of the DPCIM. The control and treated DPCIM were evaluated by various analytical techniques such as X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer (PSA), surface area analyzer and ultra violet-visible spectroscopy (UV-vis) analysis. The XRD analysis revealed a decrease in intensity of XRD peaks of the treated sample as compared to the control. The crystallite size of the treated DPCIM (81.02 nm) was decreased with respect to the control sample (84.99 nm). The DSC analysis showed a slight decrease in melting temperature of the treated sample. Additionally, the latent heat of fusion of treated sample was changed by 45.66% as compared to the control sample. The TGA analysis showed an increase in onset degradation temperature of the treated sample (182ºC) as compared to the control sample (142ºC). This indicated the increase in thermal stability of the treated DPCIM. PSA results demonstrated an increase in average particle size (d50) and size showed by 99% of particles (d99) by 19.2 and 40.4%, respectively as compared to the control sample. The surface area analyzer showed a decrease in surface area of treated DPCIM by 13.4%, which was well supported by the particle size results. UV spectra of the treated sample showed the disappearance of absorption peak 261 nm in treated sample as compared to the control. Overall, the result showed that biofield energy treatment has a paramount influence on physical, thermal and spectral properties of DPCIM. Therefore, it is assumed that biofield treated DPCIM could be used as a better medium for plant tissue culture applications.

Physicochemical and Spectroscopic Properties of Biofield Energy Treated Protose

Abstract

Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the control and treated samples were evaluated using various analytical techniques. The X-ray diffractograms (XRD) of control and treated samples showed the halo patterns peak that suggested the amorphous nature of both the samples of protose. The particle size analysis showed about 12.68% and 90.94 increase in the average particle size (d50) and d99 (particle size below which 99% particles are present) of treated protose with respect to the control. The surface area analysis revealed the 4.96% decrease in the surface area of treated sample as compared to the control sample. The differential scanning calorimetry (DSC) analysis revealed the 22.49% increase in the latent heat of fusion of treated sample as compared to the control. Thermogravimetric analysis (TGA) analysis showed increase in maximum thermal degradation temperature (Tmax) by 5.02% in treated sample as compared to the control. The increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. Fourier transform infrared (FT-IR) study showed the alteration in the vibrational frequency of functional groups like N-H, C-H, and S=O of treated protose as compared to the control sample. Based on the overall analytical results, it is concluded that Mr. Trivedi’s biofield energy treatment has a significant impact on the physicochemical and spectral properties of protose. As a result, the treated protose might be more effective as a culture medium than the corresponding control.

Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium

Abstract

SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently,the control and treated samples were analyzed using various analytical techniques. The CHNO analysis showed about 2.16, 4.87, and 5.89% decrease in percent contents of carbon, hydrogen, and oxygen, respectively; while 9.49% increase in nitrogen contents of treated sample as compared to the control. X-ray diffraction (XRD) analysis showed 7.23% decrease in crystallite size of treated sample as compared to the control. The thermogravimetric analysis (TGA) analysis showed the increase in onset temperature of thermal degradation by 19.61% in treated sample with respect to the control. The control sample showed the 48.63% weight loss during the thermal degradation temperature (Tmax) while the treated sample showed only 13.62% weight loss during the Tmax. The differential scanning calorimetry (DSC) analysis showed the 62.58% increase in the latent heat of fusion of treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated SFRE-M showed the alteration in the wavenumber of C-O, C-N and C-H vibrations in the treated sample as compared to the control. Altogether, the XRD, TGA-DTG, DSC, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal and spectral properties of SFRE-M. The treated SFRE-M was more thermal stable than the control SFRE-M and can be used as the better culture media for mammalian cell culture.

Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment

Abstract

Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away®system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.

Biochemical Differentiation and Molecular Characterization Of Biofield Treated Vibrio parahaemolyticus

Abstract

The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, whereas, Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment of Gr. IIB on day 142 (Study II), the sample was divided into three separate tubes. The tubes first, second and third were analyzed on day 5, 10, and 15, respectively. The biochemical reaction and biotyping were performed using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of V. parahaemolyticus with other bacterial species after the treatment. The results of biochemical reactions were altered 24.24%, out of thirty-three in the treated groups with respect to the control. Moreover, negative (-) reaction of urea was changed to positive (+) in the revived treated Gr. IIB, Study II on day 15 as compared to the control. Besides, biotype number was substantially changed in all the treated groups as compared to the control. However, change in organisms were reported in Gr. IIA on day 10 and in Gr. IIB; Study II on day 5 as Shewanella putrefaciens and Moraxella/Psychrobacter spp., respectively with respect to the control i.e. Vibrio sp. SF. 16S rDNA analysis showed that the identified sample in this experiment was V. parahaemolyticus after biofield treatment, and the nearest homolog genus-species was observed as Vibrio natriegens with 98% gene identity. The results envisaged that the biofield energy treatment showed an alteration in biochemical reaction pattern and biotype number on the strain of V. parahaemolyticus.

Use of Energy Healing Medicine Against Escherichia coli for Antimicrobial Susceptibility, Biochemical Reaction and Biotyping

Abstract

Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobial sensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates. The minimum inhibitory concentration (MIC) study showed the alteration in MIC values of about 34.37% (11 out of 32) tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Piperacillin/tazobactam was reported with improved sensitivity and four-fold decrease in the MIC value (64 to ≤16 μg/mL) in LS 42, as compared with the control. Amoxicillin/kclavulanate reported with improved sensitivity pattern from resistance to susceptible, with two-fold decrease in MIC value (>16/8 to ≤8/4 μg/mL) in biofield treated LS 51. Further, biochemical study showed 24.24% alteration (8 out of 33) in tested biochemical reactions after treatment among four isolates of E. coli as compared to the control. A change in biotype number (7774 4272) was reported as compared to the control, (7311 4012), with new organism identified as Klebsiella pneumoniae in biofield treated LS 13 with respect to the control organism, E. coli. Overall, data suggested that Mr. Trivedi’s biofield energy treatment can be applied to alter the antimicrobial sensitivity, biochemical reactions and biotype number of E. coli.

In vitro Evaluation of Biofield Treatment on Viral Load Against Human Immunodeficiency-1 and Cytomegalo Viruses

Abstract

Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of AIDS. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer. Viral load (HIV-1 RNA and HCMV DNAaemia) was considered as surrogate marker for assessment of the impact of Mr. Trivedi’s biofield treatment in HIV infected stored plasma samples. The viral load quantification of HIV-1 RNA in infected stored plasma samples was significantly reduced by 65% in biofield treated group as compared to control. Additionally, viral load of HCMV DNAaemia in infected stored plasma samples was also reduced by 80% in the biofield treated group as compared to control. Because, children are more prone to HCMV infection and adults are generally liable to suffer from HIV-1 infection. As the biofield treatment has reduced HCMV DNAaemia, it could be beneficial for HIV infected children populations. Altogether, data suggest that biofield treatment has significantly reduced the viral load quantification in HIV-1 and HCMV infected stored plasma samples and could be a suitable alternative treatment strategy for AIDS patients in near future.

Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri

Abstract

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.

Improved Susceptibility Pattern of Antimicrobials Using Vital Energy Treatment on Shigella sonnei

Abstract

Complementary and alternative medicine (CAM) has become increasingly popular and reported for countless benefits in biomedical health care systems. The study assessed the potential impact of The Trivedi Effect® (biofield energy) on Shigella sonnei for changes in antimicrobial sensitivity, biochemical study, and biotype number using MicroScan Walk-Away® system. The cells were obtained from MicroBioLogics Inc., USA bearing the American Type Culture Collection (ATCC 9290) number, and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was subjected to Mr. Trivedi’s biofield energy treatment and further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 160 (Study I). The Gr. IIB sample was retreated on day 160 (Study II), and was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15, respectively. Results showed that 35% (7 out of 20) antimicrobials were reported with improved sensitivity profile. Moreover, the minimum inhibitory concentration study showed that 56.25% (18 out of 32) tested antimicrobials were reported with decreased concentration by two to four-fold as compared with the control after biofield treatment. The effect was further analyzed and sustained in the biochemical study, where 57.57% (19 out of 33) tested biochemicals showed altered reaction pattern as compared with the control. The biotype study showed an alteration in the biotype number in all the experimental treated groups as compared to the control. Encouraging results suggests that bioenergy healing treatment as an integrative medicine against S. sonnei would be a better and safe treatment approach in near future.

Antibiogram of Biofield-Treated Shigella boydii: Global Burden of Infections

Abstract

Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydiii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9207) strain of S. boydiii was used in this experiment. The study was conducted in revived and lyophilized state of S. boydiii. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. boydiii were subjected to Mr. Trivedi’s biofield treatment. Gr. II was assessed on day 5 and day 10, while Gr. III on day 10 with respect to control (Gr. I). Sensitivity pattern of amoxicillin/k-clavulanate was improved from intermediate (I) to susceptible (S) with correspond to MIC value was also reduced by two folds (16/8 to ≤ 8/4 μg/mL) in both the treated groups as compared to control. The antimicrobial susceptibility of S. boydiii showed 15% alteration in Gr. II on day 5, while significant (40%) alteration was found on day 10 as compared to control. The MIC values of antimicrobials for S. boydiii also showed 12.50% alteration in Gr. II on day 5 while, significant alteration (59.38%) of minimum inhibitory concentration (MIC) value was found in Gr. II on day 10 as compared to control. It was observed that overall 69.70% biochemical reactions were changed in which 66.67% alteration was found in Gr. II on day 10 with respect to control. Moreover, biotype numbers were changed in all the treated groups without alteration of organism as compared to control. These results suggested that biofield treatment had significant impact on S. boydiii in Gr. II on day 10 with respect to antimicrobial susceptibility, MIC and biochemical reactions pattern.

Evaluation of Plant Growth Regulator, Immunity and DNA Fingerprinting of Biofield Energy Treated Mustard Seeds (Brassica juncea)

Abstract

Among the oilseeds grown around the world, mustard is one of the important crop worldwide due to its wide adaptability and high yielding capacity. Owing to the importance of its utilities as condiment, cooking oil and some medical aids, the demand for its seed production is too high. The present study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on mustard (Brassica juncea) for its growth-germination of seedling, glutathione (GSH) content in leaves, indole acetic acid (IAA) content in shoots and roots and DNA polymorphism by random amplified polymorphic-DNA (RAPD). The sample of B. juncea was divided into two groups. One group was remained as untreated and coded as control, while the other group was subjected to Mr. Trivedi’s biofield energy treatment and referred as the treated sample. The growth-germination of B. juncea seedling data exhibited that the biofield treated seeds were germinated faster on day 5 as compared to the control (on day between 7-10). The shoot and root length of seedling were slightly increased in the treated seeds of 10 days old with respect to untreated seedling. Moreover, the major plant antioxidant i.e. GSH content in mustard leaves was significantly increased by 206.72% (p<0.001) as compared to the untreated sample. Additionally, the plant growth regulatory constituent i.e. IAA level in root and shoot was increased by 15.81% and 12.99%, respectively with respect to the control. Besides, the DNA fingerprinting data using RAPD revealed that the treated sample showed an average 26% of DNA polymorphism as compared to the control. The overall results envisaged that the biofield energy treatment on mustard seeds showed a significant improvement in germination, growth of roots and shoots, GSH and IAA content in the treated sample. In conclusion, the biofield energy treatment of mustard seeds could be used as an alternative way to increase the production of mustard.

Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (Gossypium hirsutum L.)

Abstract

Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.

Agronomic Characteristics, Growth Analysis, and Yield Response of Biofield Treated Mustard, Cowpea, Horse Gram, and Groundnuts

Abstract

Mustard, cowpea, horse gram, and groundnuts are the seasonal pulse crops used as food and fodder in many regions of the world. In the present study, the impact of biofield energy treatment on mustard, cowpea, horse gram, and groundnuts were studied with respect to overall growth, yield, and its related yield attributes. Seeds of each crop was selected and divided in two groups, i.e. control and treated. The treated group of each seed crops was subjected to Mr. Trivedi biofield energy treatment, and were plotted in the separate fields. The plot with untreated seeds were provided with all the precautionary measures such as pesticides, fungicides and organic additives, while no such measures were taken in the plot with treated seeds. Both group of crops were further analyzed and compared for growth, yield, and yield attributes. Further, the effect of biofield treatment was also evaluated on horse gram using Random Amplified Polymorphic DNA (RAPD) analysis in order to determine their epidemiological relatedness and genetic characteristics. The results suggest that the percentage increase in yield was maximum in mustard (500%), followed by horse gram (105%), cow pea (52%), and groundnut (44%) as compared with their control. However, improved plant height, overall growth, yield of seeds, plants were free from any diseases and pest were observed in treated group as compared with its respective control. RAPD analysis using eight primers results in polymorphism and the percentage of true polymorphism observed between control and treated samples of horse gram seed sample with an average value of 53%. The overall results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on mustard, cowpea, horse gram, and groundnuts, which might be used as a better alternative approach to increase the yield of crop as compared with the synthetic chemicals.

In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi

Abstract

Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Mini-API ID32C strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell from S→I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs: fluconazole from S→I (T1 on day 10) and itraconazole S→I (T1 and T2 on all assessment days). The Cryptococcus neoformans changed from S→I in T1 cell on day 5 and 10, against itraconazole. Sensitivity of Candida tropicalis was also altered from I→R against flucytosine (T1 and T2, on all assessment days). Similarly, Saccharomyces cerevisae altered from S→I (T1) and S→R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8 folds, as compared to the control. Fungal identification data showed the significant changes in species similarity of few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10. C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day 5) and 99.0% (T2). These findings suggest that biofield treatment could be applied to alter the susceptibility pattern of antifungal drug therapy in future.

Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus

Abstract

Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.

Antibiogram Typing of Biofield Treated Multidrug Resistant Strains of Staphylococcus Species

Abstract

Antimicrobial resistance is a global health issue in the developing countries. This study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant (MDR) clinical lab isolates (LSs) of Staphylococcus species viz. Staphylococcus haemolyticus (LS 18), Staphylococcus epidermidis (LS 21), and Staphylococcus aureus (LS 30). Each strain was divided into the two groups i.e.control and treated. The control and treated groups were analyzed for the antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical analysis and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with the control group. The sensitivity of erythromycin was improved from resistant to susceptible, while levofloxacin sensitivity was also improved from intermediate to susceptible in LS 21 isolate. The MIC results showed a decrease in the concentrations of ceftriaxone, erythromycin, imipenem, and levofloxacin antimicrobials in LS 21 as compared to the control. Linezolid and vancomycin also showed decrease in MIC as compared to the control in LS 30. Overall, 20.69% antimicrobials showed decrease in MIC value out of the tested twenty-nine after biofield treatment in Staphylococcus species. The biochemical study showed a 25% alteration in biochemical reactions as compared to the control. A significant change was reported in biotype numbers for all the three strains of MDR Staphylococcus species after biofield treatment as compared to the respective control group. On the basis of changed biotype number (306366) after biofield treatment in LS 18, the new organism was identified as Staphylococcus simulans with respect to the control species i.e. Staphylococcus haemolyticus (302302). The control group of S. epidermidis and S. aureus showed biotype number as 303064 and 757153 respectively. After biofield treatment, LS 21 and LS 30 isolates showed altered biotype number as 307064 and 317153 respectively. Overall, results conclude that biofield treatment could be used as complementary and alternative treatment strategy against multidrug resistant strains of Staphylococcus species with improved sensitivity and reduced MIC values of antimicrobial.

Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa

Abstract

Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 1014% (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to %0% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.

Assessment of Antibiogram of Multidrug-Resistant Isolates of Enterobacter aerogenes after Biofield Energy Treatment

Abstract

Enterobacter aerogenes (E. aerogenes) has been reported as the versatile opportunistic pathogen associated with the hospital infections worldwide. The aim of the study was to determine the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant clinical lab isolates (LSs) of E. aerogenes. The MDR isolates of E. aerogenes (i.e., LS 45 and LS 54) were divided into two groups, i.e., control and treated. Samples were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study, and biotype number using MicroScan Walk-Away®system, on day 10 after the biofield treatment. The antimicrobial sensitivity assay showed 14.28% alteration out of twenty eight tested antimicrobials with respect to the control. The cefotetan sensitivity changed from intermediate (I) to inducible β-lactamase (IB), while piperacillin/tazobactam changed from resistant to IB in the treated LS 45. Improved sensitivity was reported in tetracycline, i.e., from I to susceptible (S) in LS 45, while chloramphenicol and tetracycline sensitivity changed from R to I in treated LS 54. Four-fold decrease in MIC value was reported in piperacillin/tazobactam, and two-fold decrease in cefotetan and tetracycline in the biofield treated LS 45 as compared to the control. MIC results showed an overall decreased MIC values in 12.50% tested antimicrobials such as chloramphenicol (16 μg/mL) and tetracycline (8 μg/mL) in LS 54. The biochemical study showed an overall 45.45% negative reaction in the tested biochemical in both the treated isolates as compared to the control. A change in biotype number was reported in MDR isolates (LS 45 and LS 54), while in LS 54, altered biotype number, i.e., 0406 0374 as compared to the control (7770 4376), with identification of the new species as Stenotrophomonas maltophilia with brown color as special characteristic. The study findings suggest that Mr. Trivedi’s biofield energy treatment on clinical MDR isolates of E. aerogenes has the significant effect on altering the sensitivity of antimicrobials, decreasing the MIC values, changed biochemical reactions, and biotype number.

Characterization of Antimicrobial Susceptibility Profile of Biofield Treated Multidrug-resistant Klebsiella oxytoca

Abstract

Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Control group remain untreated and treated group was subjected to Mr. Trivedi’s biofield. The analysis was done on day 10 after biofield treatment and compared with control group. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical reactions and biotype number using MicroScan Walk-Away® automated system. Experimental results showed the impact of biofield treatment on K. oxytoca and found alteration in both antimicrobial sensitivity and MIC values as compared with untreated group. Antimicrobial sensitivity of about 26.67% tested antimicrobials out of thirty was altered with respect to control. MIC results showed about 12.50% alterations in tested antimicrobials as compared to control. Biochemical study showed 24.24% alteration in tested biochemical reactions after biofield treatment. A significant change in biotype number (7713 5272) was identified after biofield treatment as compared to control (7775 4332). In treated group, a new species was identified as Kluyvera ascorbata, as compared to control, K. oxytoca. Study findings suggest that biofield treatment has a significant effect in altering the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug resistant strain of K. oxytoca. Biofield treatment could be applied to alter the antibiogram-resistogram pattern of antimicrobials.

Antimicrobial Sensitivity, Biochemical Characteristics and Biotyping of Staphylococcus saprophyticus: An Impact of Biofield Energy Treatment

Abstract

Staphylococcus saprophyticus (S. saprophyticus) is a frequent cause of urinary tract infection in the young women. The current study was designed to analyze the effect of biofield energy treatment on S. saprophyticus for evaluation of its antibiogram profile, biochemical reactions pattern and biotyping characteristics. Two sets of ATCC samples were taken in this experiment and denoted as A and B. Sample A was revived and divided into two parts Group (Gr.I) (control) and Gr.II (revived); likewise, sample B was labeled as Gr.III (lyophilized). Gr. II and III were given with Mr. Trivedi’s biofield energy treatment. The control and treated groups of S. saprophyticus cells were tested with respect to antimicrobial susceptibility, biochemical reactions pattern and biotype number using MicroScan Walk-Away® system. The 50% out of twenty-eight tested antimicrobials showed significant alteration in susceptibility and 36.67% out of thirty antimicrobials showed an alteration in minimum inhibitory concentration (MIC) value of S. saprophyticus in revived treated cells (Gr. II, day 10), while no alteration was found in lyophilized treated cells (Gr. III, day 10) as compared to the control. It was also observed that overall 14.81%, out of twenty-seven biochemical reactions were altered in the revived treated group with respect to the control. Moreover, biotype number was changed in Gr. II, on day 5 (246076) and in Gr. III, on day 10 (242066), while organism along-with biotype number was also changed in Gr. II, on day 10 (342066, Staphylococcus hominis subsp. novobiosepticus) as compared to the control (242076, S. saprophyticus). The result suggested that biofield treatment has the significant impact on S. saprophyticus in revived treated cells with respect to the antimicrobial susceptibility, MIC, biochemical reactions pattern and biotype.

Effect of Biofield Treated Energized Water on the Growth and Health Status in Chicken (Gallus gallus domesticus)

Abstract

The current study was attempted to investigate the effect of Mr. Trivedi’s biofield energy treated energized water on chicken. The total 4200 chicks were equally divided into two groups i.e. control and treated. The biofield treated energized water was provided to the treated chicks, while the control chicks were drunk with standard drinking water. During the experiment the parameters such as mortality, body weight, food intake etc. were assessed in both control and energized water treated birds. The mortality rate was reduced in the energized water treated chicks as 54.55% in week 1, 42.11% in week 6, and 39.13% in week 4, as compared to the control chicks. Moreover, the average body weight was increased by 12.50% in week 1 as compared to the control chicks. The feed conversion ratio was gradually decreased which indicated that the energized water treated chicks took less feeds while the body weight was increased in comparison to the control chicks. Besides, the energized water treated birds showed statistically significant (p<0.007) with 15.47% increase in the edible meat weight as compared to the control chicks. Moreover, the feather, skin and internal organ weight were significantly reduced by 21.22% (p<0.001) of energized water treated chicks as compared to the untreated chicks. The protein content was increased by 10.11% and cholesterol was decreased by 4.64% in birds of the treated group as compared to the control. The European efficiency factor was also increased by 10.67% in the energized water treated birds as compared to the control chicks.. The European efficiency factor was also increased by 10.67% in the energized water treated birds as compared to the control chicks. Altogether, the results suggest that Mr. Trivedi’s biofield treated energized water could be a cost effective feeding approach in chicken production.

Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

Abstract

Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes. First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to susceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the treated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration in the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA (53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus) on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and (53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed that the identified microbe as Citrobacter freundii(GenBank Accession Number: DQ517285) with 95% identity. The nearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). The results suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state.

Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis

Abstract

Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield energy treatment on N. otitidis and analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing. The strain of N. otitidis (ATCC 14630) was divided into two parts, control and treated. Antimicrobial susceptibility was studied using the broth microdilution technique. Overall, the MIC values of 16.67% antimicrobials were changed in the treated group of N. otitidis as compared to the control. Moreover, MIC value of trimethoprim/sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 μg/mL) in the biofield energy treated sample as compared to the control without alteration in the sensitivity spectrum. The 16S rDNA analysis showed that the treated sample was detected as Enterobacter aerogenes strain NCTC10006T (GenBank Accession No: AJ251468) with 98% identity of gene sequencing data. However, the nearest homolog genus-species was found as Kluyvera cryocrescens (GenBank Accession No: AM184245). Using RAPD biomarkers, the sample showed an average range of 34 to 53% of polymorphism among treated samples as compared to the control. The 16S rDNA sequencing of treated sample was carried out to correlate the phylogenetic relationship of N. otitidis with other bacterial species. These results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on N. otitidis.

Antimicrobial Susceptibility, Biochemical Characterization and Molecular Typing of Biofield Treated Klebsiella pneumoniae

Abstract

Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedi’s biofield energy treatment and analyzed for the antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotyping using automated MicroScan Walk-Away®system. Further, the effect of biofield treatment was also evaluated using Random Amplified Polymorphic DNA (RAPD) in order to determine their epidemiological relatedness and genetic characteristics of biofield treated K. pneumoniaesamples. The antimicrobial susceptibility results showed an improve sensitivity (i.e. from intermediate to susceptible) of ampicillin/sulbactam and chloramphenicol, while altered sensitivity of cephalothin (i.e. from susceptible to intermediate) was also reported as compared to the control sample. The MIC value showed two-fold decrease in MIC value of ampicillin/sulbactam (i.e. 16/8 to ≤8/4 μg/mL) and chloramphenicol (i.e. 16 to ≤ 8 μg/mL) as compared to the control. The cephalothin showed two-folds change (i.e. ≤ 8 to 16 μg/mL) in the MIC value as compared with the control. Biofield treatment showed 9.09% alterations in biochemical reactions followed by a change in biotype number (7774 4272) in the treated group with respect to the control (7774 4274). Genetic fingerprinting was performed on control and treated samples using RAPD-PCR biomarkers, which showed an average range of 11 to 15% of polymorphism among the treated samples with respect to the control. These results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on K. pneumoniae.

Antibiogram Pattern of Shigella flexneri: Effect of BioField Treatment

Abstract

Shigellosis is a major public health burden in India and its neighboring countries due to infection of Shigella species. The current study was attempted to investigate the effect of biofield treatment on Shigella flexneri (S. flexneri) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9199) strain of S. flexneri was used in this experiment. The study was conducted in revived and lyophilized state of S. flexneri. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. flexneri were subjected to Mr. Trivedi’s biofield treatment. Gr. II was assessed on day 5 and day 10, while Gr. III on day 10 after biofield treatment with respect to control (Gr. I). The antimicrobial susceptibility of S. flexneri showed 35% alteration in Gr. II on day 10 while no alteration were observed on day 5 (Gr. II) and in Gr. III as compared to control. The minimum inhibitory concentration (MIC) values of biofield treated S. flexneri also showed significant (46.88%) alteration in Gr. II on day 10 while no alteration were observed on day 5 (Gr. II) and in Gr. III as compared to control. It was observed that overall 24.24% biochemical reactions were altered in which 21.21% alteration was found in Gr. II on day 10 with respect to control. Moreover, biotype number was changed in Gr. II on day 10 with identification of new organism i.e. Edwardsiella tarda (40015042) as compared to untreated strain of Shigella species (40010000). The result suggested that biofield treatment has significant impact on S. flexneri in revived treated cells (Gr. II) on day 10 with respect to antimicrobial susceptibility, MIC, biochemical reactions pattern and biotyping.

Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii

Abstract

Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols until needed for experiments. M. morganii strain was divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment on day 142, the sample (Study II) was divided into three separate tubes. First, second and third tube was further analyzed on day 5, 10 and 15 respectively. All experimental parameters were studied using the automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of M. morganii with other bacterial species. Antimicrobial susceptibility results showed 32.14% alterations, while minimum inhibitory concentration results showed 18.75% alterations of the tested antimicrobials. Biochemical study also showed altered positive reactions in nitrofurantoin and indole with respect to control. Biotype study showed alteration in Gr. IIB, study II, on day 15 (4005 1446) as compared to the control (4004 1446). 16S rDNA sequencing analysis showed similar results with the identified microbe as M. morganii (GenBank accession number: AB210972) having 80% identity of the gene sequencing data. Total 1507 base nucleotide of 16S rDNA gene sequences were analyzed by multiple alignments, while nearest homolog genus-species of M. morganii was found as Providencia rettgeri (accession number: AM040492). These results suggested that biofield treatment has a significant impact on M. morganii in lyophilized as well as revived state.

Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes

Abstract

Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedi’s biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away® system. In addition, treated group of E. aerogenes was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of E. aerogenes with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among twenty-eight tested antimicrobials. Similarly, 15.65% tested antimicrobials showed an alteration in MIC values. Chloramphenicol showed improved sensitivity i.e. resistant to susceptible after biofield treatment, with the support of decreased MIC by two folds (i.e. >16 to ≤8 µg/mL). Norfloxacin also showed decrease MIC by two folds (i.e. 8 to ≤4 µg/mL) as compared to control. Biofield treatment showed an impact on biochemical reactions (9.09%) followed by a change in biotype number (7770 5272) in treated group with respect to control (7770 5372). Using RAPD analysis, sample showed an average range of 4 to 42% of polymorphism, while 16S rDNA study showed that treated sample was detected as Kluyvera cryocrescens (GenBank Accession Number: AM184245) with 97% identity of gene sequencing data, which was nearest homolog species to Enterobacter aerogenes strain: C1111 (Accession No. AB244467). These results suggest that Mr. Trivedi’s unique biofield treatment can alter the antimicrobial sensitivity pattern, thus it can be used as alternate energy medicine in future.

Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen

Abstract

Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains of S. agalactiae gr. B were selected for the study. Gr. I was considered as control. Both revived (Group; Gr. II) and lyophilized (Gr. III) strains of S. agalactiae gr. B were subjected to Mr. Trivedi’s biofield treatment. Gr. II was assessed on day 5 and day 10 while Gr. III on day 10 with respect to the control (Gr. I) using MicroScan Walk-Away® system. Although biofield treatment did not show any change with respect to susceptibility pattern. However the minimum inhibitory concentration of S. agalactiae gr. B showed significant (70.37%) alteration, out of twenty-seven tested antimicrobials, among which in Gr. II i.e. 62.96% on day 5 and 66.67% on day 10 while no alteration was found in lyophilized group (Gr. III) as compared to the control. Moreover, the improvement of MIC value of norfloxacin was observed by two-fold (8 to ≤4 μg/mL) in Gr. II on day 10 after biofield energy treatment as compared to the control. It was observed that overall 48.28% biochemical reactions, out of twenty-nine were altered in Gr. II with respect to the control. Moreover, biotype numbers were changed in Gr. II on day 5 (777777615) and on day 10 (757677405) as compared to the control (237147047). The results suggest that biofield treatment has significant impact on S. agalactiae gr. B in revived treated cells (Gr. II) with respect to MIC values, biochemical reactions pattern and biotype number

Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Abstract

Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi’s unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.

Investigation of Biofield Treatment on Antimicrobial Susceptibility, Biochemical Reaction Pattern and Biotyping of Enteropathogenic Multidrug-Resistant Escherichia coli Isolates

Abstract

Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli.

Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Control and treated samples were identified with respect to its antimicrobial sensitivity assay, biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with its respective control group.

Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. MIC results showed the alteration in MIC of about 40.63% antimicrobials out of thirty two tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Ticarcillin/k-clavulanate showed improved sensitivity (R → I) with decreased MIC value in LS 9 as compared to control. A fourfold and twofold decreased in MIC values were reported in case of piperacillin/tazobactam (in LS 9) and chloramphenicol (in LS 8), respectively as compared to respective control. Biochemical study showed a 39.39% alteration in biochemical reactions after treatment among four isolates of E. coli as compared to control. A significant change in biotype numbers were reported in three clinical isolates (i.e. LS 8, LS 9, and LS 11) of MDR E. coli as compared to control. On the basis of changed biotype number (7774 5272) after biofield treatment, organism with maximum probability was identified as Enterobacter aerogenes in LS 8 as compared to control, (E. coli, 7711 5012).

Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. coli.

Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

Abstract

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into two groups i.e. control and biofield treated. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of MDR K. pneumonia isolates. MIC results showed an alteration in 30% of tested antimicrobials out of thirty after biofield treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in biochemical reactions as compared to control. A significant change in biotype numbers were reported in all four clinical isolates of MDR K. pneumoniae after biofield treatment as compared to control group. On the basis of changed biotype number after biofield treatment, new organism was identified as Enterobacter aerogenes in LS 2 and LS 14. These results suggest that biofield treatment has a significant effect on altering the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment

Abstract

Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S. paratyphi A strain were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 9150). The study was conducted in revived and lyophilized state of S. paratyphi A. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. paratyphi A were subjected to Mr. Trivedi’s biofield treatment. Revived treated cells was assessed on day 5 and day 10, while lyophilized treated cells assessed on day 10 after biofield treatment with respect to control (Gr. I). The antimicrobial susceptibility of S. paratyphi A showed significant (60%) alteration in revived treated cells (Gr. II) on day 10 as compared to control. The MIC values of S. paratyphi A also showed significant (53.12%) alteration in Gr. II and on day 10 while, no alteration was found in Gr. on day 5 as compared to control. It was observed that overall 18.18% biochemical reactions were altered in the treated groups with respect to control. Moreover, biotype numbers were substantially changed in Gr. II, on day 5 (53001040, S. paratyphi A), on day 10 (57101050, Citrobacter freundii complex) as compared to control (53001000, S. paratyphi A). Besides, biotype number was also changed in Gr. III (53001040, S. paratyphi A) as compared to control. The overall result suggested that biofield treatment had significant impact on S. paratyphi A in Gr. II on day 10 with respect to antimicrobial susceptibility, MIC values and biotype number.

Antimicrobial Susceptibility of Proteus mirabilis: Impact of Biofield Energy Treatment

Abstract

Proteus mirabilis (P. mirabilis) is widespread in nature, mainly found in soil, water, and the flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield energy treatment on P. mirabilis both in lyophilized as well as revived state for antimicrobial susceptibility, biochemical characteristics, and biotype. P. mirabilis cells were procured from MicroBioLogics Inc., USA, in a sealed pack bearing the American Type Culture Collection (ATCC 25933) number and stored according to the recommended storage protocol until needed for experiments. Two sets of ATCC samples were taken in this experiment and denoted as A and B. The ATCC A sample was revived and divided into two parts Gr.I (control) and Gr.II (revived); likewise, the ATCC B was labeled as Gr.III (lyophilized). Group II and III were given with biofield treatment. All experimental parameters were studied using automated MicroScan Walk-Away® system. The result of antimicrobial susceptibility and minimum inhibitory concentration showed 6.67% and 9.38% alteration, respectively in treated cells of P. mirabilis as compared to the control. In addition, the overall biochemical reactions were significantly altered (42.42%) in the treated groups with respect to the control. Moreover, biotype number was changed in the treated cells, Gr. II, day 5 (40061546) and day 10 (77365764), while without alteration of organism as compared to the control (40061544; Proteus mirabilis). The results suggested that biofield treatment has an impact on P. mirabilis in revived state predominately.