An Alternative Therapeutic Approach on Bone Cells Differentiation after Administration of Biofield Energy Treated Vitamin D3 in MG-63 Cell line

The objective of the current experiment was to evaluate the potential of Consciousness Energy Healing-based test items (Vitamin D3 and DMEM) on bone parameters. Both the TI were distributed into two individual parts. One part of each samples was received Consciousness Energy Healing Treatment by Eileen Mary Meagher and those samples were labelled as the Biofield Treated (BT) samples, while the other parts of each samples were denoted as the untreated test items (UT). Cell viability data showed more than 71% viable cells signifies a safe and nontoxic profile of test items on MG-63 cell line. ALP was significantly increased by 132.18% and 126.52% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 50 µg/mL; while 139.06% (at 0.1 µg/mL) in UT-DMEM+BT-TI compared to untreated. Collagen was significantly increased by 172.76% at 1 µg/mL in UT-DMEM+BT-TI, while 89.65% in BT-DMEM+UT-TI at 10 µg/mL than untreated. Additionally, collagen was significantly increased by 111.2% in BT-DMEM+UT-TI at 50 µg/mL, while 113.16% in BT-DMEM+BT-TI at 100 µg/mL than untreated. The percent of bone mineralization was significantly increased by 206.21%, 168.74%, and 224.82% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared with the untreated. In addition, percent of bone mineralization data showed a significant increased by 190.63%, 181.07%, and 245.41% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 1 µg/mL than untreated. Moreover, at 10 µg/mL it was significantly increased by 129.44%, 111.76%, and 109.81% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 10 µg/mL with respect to untreated. Based on the study outcomes it emphasized that Biofield Energy Treatment could regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases.

Cell viability using MTT assay with more than 71% among the tested groups, which suggest that test samples are found as safe and nontoxic. ALP level was increased by 132.18% and 126.52% in the BT-DMEM+UT-TI and BT-DMEM+UT-TI groups, respectively at 50 µg/mL while 139.06% (at 0.1 µg/mL) in the UT-DMEM+BT-TI groups compared to the untreated test item and DMEM group. Collagen level was significantly increased by 34.48% and 89.65% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL; while 172.76% at 1 µg/mL in the UT-DMEM+BT-TI groups compared to the untreated test item and DMEM group. In addition, 46.53% and 111.2% increased the level of collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 50 µg/mL, while 113.16% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated test item and DMEM group. Besides, the bone mineralization percent was significantly increased by 206.21%, 168.74%, and 224.82% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 0.1 µg/mL as compared with the untreated group. In addition, the percent of bone mineralization data showed a significant increased by 190.63%, 181.07%, and 245.41% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 1 µg/mL compared with the untreated group. Moreover, at 10 µg/mL the percentage of bone mineralization was significantly increased by 129.44%, 111.76%, and 109.81% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 10 µg/mL with respect to the untreated test item and DMEM group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing-based vitamin D3 might be a suitable nutritional supplement for the management of various bone-related disorders viz. rickets, osteoporosis, stress, aging, Paget’s disease of bone, osteoma, osteomalacia, bone and/or joint pain, bone loss and fractures, hormonal imbalance, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Further, Biofield Energy Treated vitamin D3 can be useful as anti-aging, anti-inflammatory, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, roles. Besides, it can also be utilized in organ transplants like kidney, liver and heart transplants, hormonal imbalance, and various inflammatory conditions such as Alzheimer’s Disease, Ulcerative Colitis, Dermatitis, Irritable Bowel Syndrome, Hashimoto Thyroiditis, and also useful against immune-related disorders like Diabetes,Parkinson’s Disease, Aplastic Anemia, Pernicious Anemia, Multiple Sclerosis, Hepatitis, Graves’ Disease, Myasthenia Gravis, Atherosclerosis, Systemic Lupus Erythematosus, etc. near future.