An in Vitro Study of Biofield Energy Healing Based Herbomineral Formulation for Skin Protection

The current research work deals with the impact of the Consciousness Energy Healing Treatment (The Trivedi Effect®) based herbomineral test formulation and cell medium (DMEM) against various skin health parameters. The test formulation is the combination of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, i.e. one part received the Biofield Energy Treatment by Eileen Mary Meagher and was labeled as the Biofield Treated (BT) samples, while other represented was defined as the untreated test (UT) samples. The MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability. Cell proliferation assay using BrdU method showed an improved proliferation rate by 343.23%, 316.13%, and 512.75% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL, compared with the untreated group. The collagen level was significantly increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The level of elastin was increased by 47.0% and 64.1% at 2.5 and 1.25 µg/mL, respectively in the BT-DMEM + UT-Test formulation, group compared with the untreated group. Hyaluronic acid synthesis was increased by 4.37%, 41.47%, and 19.74% at 2.5, 1.25, and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation group with respect to the untreated group. Melanin synthesis was significantly inhibited by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation group compared with the untreated group. Anti-wrinkling effect showed an improved cell viability by 4.32% and 2.47% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT- Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay exhibited improved a healing rate up to 6% in the HFF-1 and HaCaT cells lines in the Biofield Energy Treated Test formulation. In conclusion, results suggest that The Trivedi Effect®- Consciousness Energy Healing Treatment based test formulation and medium have the ability to improve the skin health and can be used as skincare products cosmetics for anti-ageing, anti-wrinkling action and for the treatment of many skin diseases such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.

In the present study, cell viability was significantly improved with viability in more than 75% cells in all the tested concentrations of the test formulation such as HFF-1 (1.25 to 10 µg/mL), HaCaT (0.625 to 5 µg/mL), and B16-F10 (10 to 40 µg/mL) using MTT assay. BrdU assay showed increased cellular proliferation by 343.23%, 316.13%, and 512.75% at 17.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively. An increased cellular proliferation by 287.1%, 264.4%, and 428.9% was found in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen level was increased by 15.70% and 13.52% at 2.5 and 0.625 µg/mL respectively, in the UT-DMEM + BT- Test formulation group, with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly increased by 47.0%, 64.1%, and 11.1% in the BT-DMEM + UT- Test formulation group at 2.5, 1.25, and 0.625 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. The level of HA was significantly increased by 4.37%, 41.47%, and 19.74% in the UT-DMEM + BT-Test formulation group, at concentration 2.5, 1.25, and 0.625 µg/mL, respectively with respect to the UT-DMEM + UT-Test formulation group. Melanin levels were reduced by 8.83%, 5.78%, and 12.68% at 0.0125, 0.0625, and 0.125 µg/mL, respectively in the UT-DMEM + BT-Test formulation groups, while melanin was also decreased by 4.73% and 13.21% at 0.0125 and 0.125 µg/mL respectively, in the BT-DMEM + UT-Test formulation group compared with the UT-DMEM + UT-Test formulation group. Anti-wrinkling potential with respect to UV-B showed an increased cell viability in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups compared to the UT-DMEM + UT-Test formulation group. Wound healing scratch assay showed a significant migration of fibroblast and keratinocyte cells with an increased percentage of covered area up to 6% in HFF-1 cells, while significant change was also reported in HaCaT cells after treatment with Biofield Energy (The Trivedi Effect®) based test formulation. Overall, the Biofield Energy Treated Test formulation and DMEM can be used significantly in different pharmacological actions such as anti-wrinkling, anti-aging, skin whitening, and wound healing agent.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) treatment with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing Treatment can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.