Antiaging Potential of Consciousness Energy Healing-Based Novel Proprietary Formulation

The study objective was to evaluate the potential of Biofield Treatment on novel proprietary formulation for skin health using HFF-1, HaCaT, and B16-F10 cells. The test formulation and DMEM were divided into two parts. One part received Biofield Treatment by Gopal Nayak and defined as Biofield Treated (BT) sample, while other part was denoted as untreated (UT) sample. MTT assay showed more than 70% cell viability, suggested the test formulation were safe and nontoxic. BrdU data showed an increased cell proliferation by 170%, 170%, and 212% at 0.1 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated group. Collagen was significantly increased by 140.8% at 5 µg/mL in UT-DMEM + BT-Test formulation than untreated. Elastin was significantly increased by 233.3%, 313.3%, and 637.8% at 0.01 µg/mL in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively than untreated. Hyaluronic acid in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation was significantly increased by 191.8%, 461.0%, and 322.5%, respectively at 0.01 µg/mL than untreated. Melanin synthesis was significantly inhibited by 44.7% (10 µg/mL) and 45.2% (10 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively contrast to untreated. Anti-wrinkling activity was improved with increased cell viability by 88.9% and 92.9% at 1 and 25 µg/mL, respectively in BT-DMEM + UT-Test formulation than untreated. Cell viability was significantly increased by 97.6% and 75.16% at 0.1 and 1 µg/mL, respectively in BT-DMEM + BT-Test formulation than untreated. The UT-DMEM + BT-Test formulation showed increased cellular migration by 2722.2% (0.01 µg/mL) in HaCaT cells than untreated. Overall, result suggests that the Trivedi Effect® has shown significant beneficial effects on skin health parameters that might lead to its potential use in various aging-related disorders, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin disease

The Trivedi Effect®-Consciousness Energy Healing based novel proprietary test formulation and DMEM showed a significant increase in cell viability with more than 70% in all the three tested cell lines i.e. HaCaT, HFF-1, and B16F10. BrdU assay data showed 170%, 170%, and 212% increased the cellular proliferation (at 0.1 µg/mL) in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively while 186.7% and 152.5% increased cellular proliferation in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the UT-DMEM + UT-Test formulation group. The skin collagen level was significantly increased by 140.8% at 5 µg/mL in the UT-DMEM + BT-Test formulation group than the untreated group. Elastin level was also significantly increased by 233.3% and 212.2% at 0.01 and 1 µg/mL, respectively in the UT-DMEM + BT-Test formulation, while 313.3% and 111% at 0.01 and 1 µg/mL, respectively increased elastin in the BT-DMEM + UT-Test formulation group than untreated group. Besides, there was significantly increased elastin level by 637.8% at 0.01 µg/mL in the BT-DMEM + BT-Test formulation than untreated group. Hyaluronic acid (HA) in HFF-1 cells showed 191.8% increased at 0.01 µg/mL in the UT-DMEM + BT-Test formulation group, while 461.0% and 139.4% increased at 0.01 and 0.1 µg/mL, respectively in the BT-DMEM + UT-Test formulation group compared with the untreated group. HA also significantly increased in the BT-DMEM + BT-Test formulation group by 322.5% at 0.01 µg/mL than untreated. Melanin level was significantly reduced by 44.7% at 10 µg/mL in the UT-DMEM + BT-Test formulation group, while 45.2% decreased at 0.1 µg/mL in the BT-DMEM + UT-Test formulation group than the untreated group. Anti-wrinkling potential with respect to UV-B induced stress showed an increased cell viability at 1 µg/mL was increased by 82.6% in the UT-DMEM + BT-Test formulation group than untreated. Cell viability was significantly increased by 78.8%, 88.9%, and 92.9% at 0.1, 1, and 25 µg/mL, respectively in the BT-DMEM + UT-Test formulation group; also increased by 97.6% at 0.1 µg/mL in the BT-DMEM + BT-Test formulation group than untreated. Cellular migration was increased upto 58.55% (100 µg/mL) in the UT-DMEM + BT-Test formulation group in HFF-1 cells than the untreated group. Similarly, in HaCaT cells, the cellular migration was significantly increased by 2722.2% and 541.9% at 0.1 and 1 µg/mL, respectively in the UT-DMEM + BT-Test formulation group; while 2431.8% and 213.1% increased cellular migration at 0.1 and 1 µg/mL, respectively in the BT-DMEM + UT-Test formulation group than untreated. Similarly, the BT-DMEM + BT-Test formulation group showed an increased cell migration by 1714% and 412.7% at 0.1 and 1 µg/mL, respectively than untreated. Thus, overall experimental data suggests the significant role of Biofield Energy Healing Treatment in wound healing and other skin-related disorders such as wrinkling, aging, and skin whitening. In conclusion, the Trivedi Effect®-Consciousness Energy Healing Treated proprietary test formulation showed a significant improved skin health parameters. Besides, these results indicated that this therapy can be well used in various skin-related disorders viz. chickenpox, eczema, acne, diaper rash, warts, measles, hives, wrinkles, skin cancer, psoriasis, ringworm, rosacea, seborrheic dermatitis, rashes from allergic reactions, rashes from bacterial or fungal infections, fleshy bumps, cracked skin, scaly or rough skin, discolored patches of skin, warts, peeling skin, changes in mole color or size, a loss of skin pigment, excessive flushing, open sores or lesions, dry, ulcers, etc.