Biofield Energy Enriched Vitamin D3: A New Horizons for Development of Bone Health Using MG-63 Cells

The current study investigates the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Maire Anne Mayne and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 78% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 141.9% and 25% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI group as compared with the untreated group. In addition, 144.6% (100 µg/mL) and 120.6% (1 µg/mL) increase in ALP activity in BT-DMEM+UT-TI and BT-DMEM+BT-TI group, respectively as compared with the untreated group. The level of collagen was significantly increased by 58.7% and 72.6% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 39.7% and 101.6% at 1 and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 117.5%, 163.5%, and 169.4% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 110.2%, 116.8%, and 81% at 10, 50, and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 148.8%, 180.1%, and 129.4% at 10, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 142%, 181.1%, and 113% at 10, 50, and 100 µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

The study includes the effect of Biofield Energy Treatment on various bone health parameters. MTT assay data showed that a significant improved viability with more than 78% among the tested groups. Bone health parameters such as the level of ALP was increased by 141.9%, and 25% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI, while 144.6% at 100 µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 120.6% at 1 µg/mL. The level of collagen was significantly increased by 58.7% and 72.6% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 29.8%, 39.7%, and 101.6% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, collagen level was increased by 117.5%, 163.5%, and 169.4% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 110.2%, 116.8%, and 81% at 10, 50, and 100 µg/mL, respectively in the UT-DMEM+BT-TI group, while 148.8%, 180.1%, and 129.4% at 10, 50, and 100 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 142%, 181.1%, and 113% at 10, 50, and 100 µg/mL, respectively as compared with the untreated group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Ulcerative Colitis, Alzheimer’s Disease, Dermatitis, Irritable Bowel Syndrome, Asthma, Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Multiple Sclerosis, Aplastic Anemia, Hepatitis, Diverticulitis, Graves’ Disease, Dermatomyositis, Diabetes, Myasthenia Gravis, Parkinson’s Disease, Atherosclerosis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health, and quality of life.