Journal: American Journal of Biomedical and Life Sciences PDF
Published: 9-May-17 Volume: 5 Issue: 3 Pages: 36-46
DOI: 10.11648/j.ajbls.20170503.11 ISSN: 2330-8818 (Print) 2330-880X (Online)
Authors: Dezi Ann Koster, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Citation: Dezi Ann Koster, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana. Biofield Energy Healing Based Herbomineral Formulation: An Emerging Frontier in Cosmetic Medicine. American Journal of Biomedical and Life Sciences. Vol. 5, No. 3, 2017, pp. 36-46. doi: 10.11648/j.ajbls.20170503.11
Abstract
The application of the herbomineral formulations in general skin health are increasing day-by-day due to the excellent outcomes without any adverse effects. This study was designed to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral formulation and cell medium against various skin health parameters. The test formulation consists of minerals (zinc chloride, sodium selenate, and sodium molybdate) and L-ascorbic acid along with herbal extracts, Centella asiatica, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts, one was treated with a Biofield Treatment (BT) by Dezi Ann Koster and denoted as treated, while the other part was coded as the untreated (UT) groups. MTT assay results showed that test formulation was safe and nontoxic with more than 89% cell viability in the tested cell lines. BrdU assay showed an improved cell proliferation by 2.82% in the UT-DMEM + BT-Test formulation group compared with the untreated group. The level of collagen was significantly increased by 32.42%, 33.64%, and 29.13% at 2.5, 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation, while34.17%, 26.73%, and 17.56% increased at 2.5, 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Elastin level was increased by 408.6% at concentration of 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with untreated group. However, hyaluronic acid (HA) level was increased by 31.88%, 15.52%, and 58.29% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared with untreated group. Besides, melanin synthesis was significantly inhibited by 16.09% and 18.93% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.125 µg/mL compared with the untreated group. Anti-wrinkling activity in HFF-1 cells showed an improved cell viability by 5.49% and 11.26% at 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Wound healing scratch assay results showed a significant healing rate by 5% and 10% in HFF-1 and HaCaT cells lines, respectively with high cellular migration of fibroblast and keratinocytes. Overall, it can be concluded that the Biofield Energy Healing (The Trivedi Effect®) based test formulation and cell medium could be helpful against various skin disorders and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.
Conclusion
Herbal based products have the potential to improve various skin diseases. Currently, the world population depends on alternative health care system for curing skin related problems, although there is a movement towards seeking alternative, natural approaches for treatment. The Trivedi Effect®-Consciousness Energy Healing Treatment based novel test formulation and DMEM showed a significant outcomes, such as MTT assay displayed significant cell viability in HFF-1, HaCaT and B16-F10 cells lines with more than 89% viable cells in the Biofield Energy Healing based herbomineral test formulation. Besides, BrdU assay exhibited significant high rate of cellular proliferation in the UT-DMEM + BT-Test formulation group. Collagen synthesis was improved by 32.42%, 27.14% and 34.17% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 2.5 µg/mL compared with the UT-DMEM + UT-Test formulation group. The elastin level was found to be increased by 408.6% at concentrations 0.625 µg/mL in the UT-DMEM + BT-Test formulation group compared with the UT-DMEM + UT-Test formulation groups. However, the amount of hyaluronic acid was increased by 29.71% and 58.29% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at concentration 0.625 µg/mL compared with the UT-DMEM + UT-Test formulation group. Melanin content was inhibited by 16.09% and 18.93% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation group, respectively at 0.125 µg/mL compared with the UT-DMEM + UT-Test formulation group in B16-F10 melanoma cell line. However, the Biofield Treated Test formulation showed a significantly improved anti-wrinkling effects using UV-B induced stress in HFF-1 cells by 5.49% and 11.26% in the BT-DMEM + BT-Test formulation group at 1.25 and 0.625 µg/mL, respectively compared with the UT-DMEM + UT-Test formulation group. Wound healing scratch assay results validated significant migration of fibroblast and keratinocytes cells with increase covered area up to 10% in the HFF-1 and HaCaT cells after treatment with the Biofield Energy based test formulation and DMEM. Overall, the study results concluded that the Biofield Energy Healing based test formulation and DMEM could be used to improve various skin disorders and ageing.
Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.
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