Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health

Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.

The findings of this study suggested significantly increased cell viability with more than 75% cells in all the tested concentrations such as HFF-1, HaCaT, and B16-F10 using MTT assay suggests that the Biofield Energy Healing based herbomineral test formulation was safe and nontoxic. Besides, results of BrdU assay showed 149.13% and 118.80% increased cellular proliferation (at 8.75 µg/mL) in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, while 74.07% and 126.85% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 17.5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen level was increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL respectively, in the UT-DMEM + BT-Test formulation group, while 16.73% and 19.28% increase at 1.25 and 0.625 µg/mL respectively, in the BT-DMEM + UT-Test formulation group with respect to the UT-DMEM + UT-Test formulation group. However, the level of elastin was significantly increased by 10.38%, 14.66%, and 48.24%, in the BT-DMEM + UT-Test formulation at 2.5, 1.25, and 0.625 µg/mL respectively compared to the UT-DMEM + UT-Test formulation group. The melanin level was reduced by 11.71% and 15.75% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the UT-DMEM + UT-Test formulation group at 0.125 µg/mL. Anti-wrinkling potential with respect to UV-B showed 17.19% and 28.68% increased cell viability in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation group respectively, at 2.5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Wound healing scratch assay showed a significant migration of fibroblast and keratinocytes cells with increase covered area up to 5% in HFF-1 and HaCaT cells after treatment with Biofield Energy (The Trivedi Effect®) based test formulation. The Biofield Energy Treated Test formulation and DMEM would be a better approach for anti-wrinkling, anti-aging, skin whitening, and wound healing activity.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) treatment with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.