Effect of Biofield Energized Vitamin D3 on Bone Health in MG-63 Cell-Line

The objective of current research work was to evaluate the potential of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items like vitamin D3 and DMEM were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Faith Ann Pyka and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The MTT cell viability assay revealed that the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 191.45% in the BT-DMEM + UT-Test item (UT-TI) group at 1 µg/mL compared to the UT-DMEM + UT-TI group. Moreover, ALP level was significantly increased by 144.89% and 226.75% in the UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Further, collagen level was significantly increased by 98.15%, 80.54%, and 49.98% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the untreated group. Additionally, at 50 µg/mL level of collagen was significantly increased by 129.73%, 189.16%, and 94.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 283.74%, 27.91%, and 118.02% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Moreover, the percentage of bone mineralization was significantly increased by 265.92%, 231.82%, and 158.2% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

The cell viability assay data using MTT assay showed more than 70% cells were viable, which indicated that the test samples were safe and nontoxic in all the tested concentrations. The level of ALP was significantly increased by 144.89% and 226.75% in the UT-DMEM + BT-Test item and BT-DMEM + BT-TI groups, respectively at 10 µg/mL; while increased by 191.45% in the BT-DMEM + UT-Test item at 1 µg/mL compared to the UT-DMEM + UT-Test item group. The synthesis of collagen was significantly increased by 98.15%, 80.54%, and 49.98% in the UT-DMEM + BT-Test item, BT-DMEM + UT-TI, and BT-DMEM + BT-Test item group, respectively at 10 µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 129.73%, 189.16%, and 94.60% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + UT-TI groups, at 50 µg/mL compared to the untreated group.

Besides, the percent of bone mineralization was distinctly increased 69.19%, 62.44%, and 88.73% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the untreated group. Further, the percentage of bone mineralization was significantly increased by 283.74%, 27.91%, and 118.02% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the UT-DMEM + UT-Test item group. Moreover, the percentage of bone mineralization was significantly increased by 265.92%, 231.82%, and 158.2% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Altogether, the Biofield Energy Treated test samples (The Trivedi Effect®) demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable for the development of an alternative and more effective supplement for vitamin D3 deficiency, which could be useful for the management of various bone related disorders viz. Paget’s disease of bone, low bone density, osteoporosis, osteogenesis imperfecta, rickets, deformed bones, osteomalacia, osteoma, bone and joint pain, bone fractures, chondrodystrophia fetalis, etc. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Rheumatoid Arthritis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Scleroderma, Aplastic Anemia, Reactive Arthritis, Fibromyalgia, Sjogren Syndrome, Systemic Lupus Erythematosus, Vitiligo, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Psoriasis, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Irritable Bowel Syndrome, Atherosclerosis, Alzheimer’s Disease, Dermatitis, Diverticulitis, Hepatitis, inflammatory diseases, anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-apoptotic, anti-stress, wound healing, anti-cancer, anti-aging, anti-psychotic and anti-fibrotic action, stress management and prevention, Parkinson’s Disease etc. to modulate the immune system by improving Quality of Life (QoL).