Effect of Biofield Energy Healing (The Trivedi Effect®) Based Novel Herbomineral Formulation on Immune Biomarkers After Oral Administration in Female Sprague Dawley Rats

The herbomineral formulations usage have been increased world-wide in health care sector due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in female Sprague Dawley (SD) rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The Biofield Energy Treated group (G3) showed 6.39% increased the CD4+ count compared with the disease control (G2) group. The level of IgM was significantly increased by 23.08% and 46.15% in the G3 group and Biofield Energy Treatment group per se at day -15 (G6), respectively compared with the G2 group. The total leucocyte count (TLC) and neutrophil were significantly increased by 16.15% and 26.69% (p≤0.05), respectively in the G3 group compared with the G2 group. Further, the levels of TLC, eosinophil, and monocyte were significantly increased by 70.57% (p≤0.01), 33.33%, and 8.50%, respectively in the G6 group compared with the G2 group. Low density lipoprotein (LDL) was reduced by 6.24% in the G3 group; while triglyceride (TG) and LDL were reduced by 12.12% and 16.99%, respectively in the G6 group compared with the G2 group. The levels of serum glutamate-pyruvate transaminase (SGPT), alkaline phosphatase (ALP), and total bilirubin were significantly decreased by 20.26%, 32.98%, and 25.00%, respectively in the G3 group compared with the G2 group. Further, SGPT and total bilirubin were reduced by 9.02% and 16.67%, respectively in the G6 group compared with the G2 group. Progesterone was significantly increased by 59.85% in the G3 group, compared with the G2 group. The data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment group per se at day -15 have shown a significant immunomodulatory potential. The overall results demonstrated that the Biofield Energy Treated test formulation showed better immune response compared with the untreated test formulation. These data also suggest that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

The current results indicated that, the level of CD4+ counts was increased by 6.39% in the Biofield Energy Treated test formulation group (G3) compared with the disease control group (G2). The level of IgM was increased by 23.08% and 46.15% in the G3 group and Biofield Energy Treatment group per se at day -15 group (G6), respectively compared with the G2 group. The levels of TLC and neutrophil were significantly increased by 16.15% and 26.69% (p≤0.05), respectively in the G3 group compared with the G2 group. Moreover, the levels of TLC and eosinophil were significantly increased by 70.57% (p≤0.01) and 33.33%, respectively in the G6 group compared with the G2 group. There was a substantial reduction of the levels of TC, TG, HDL, LDL, and VLDL in the G6 group compared with the G2 group. Additionally, the levels of SGPT, ALP, and total bilirubin were significantly reduced by 20.26%, 32.98%, and 25.00%, respectively in the G3 group compared with the G2 group. The level of progesterone was significantly increased by 59.85% in the G3 group, compared with the G2 group. Therefore, it is assumed that the Biofield Energy Treated test formulation and Biofield Energy Treatment group per se at day -15 shown a significant impact on immune, cardiac, and lipid biomarkers in female Sprague Dawley rats. The Trivedi Effect®-Biofield Energy Healing Treatment administered remotely by the eighteen Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties without any adverse effect to the animals throughout the exposure period. Overall, it can be concluded that the novel Biofield Energy Treated herbomineral formulation showed better immunomodulatory action compared with the untreated test formulation against many infectious diseases viz. Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, etc. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for autoimmune disorders, anti-aging, stress prevention and management, and for the improvement of overall health and quality of life.