Effect of Biofield Energy Treated Cell Growth Medium on Human Fibroblast Cell Growth for Assessment of Skin Health Promotion Potential

Cell growth and proliferation plays an important role in wound healing, which is a complex and dynamic process. Wound management and recovery is currently done using various antiseptics and antibiotics in order to prevent or treat infection. The objective of the present study was to assess the effect of Biofield Energy (Consciousness Energy Healing Treatment - The Trivedi Effect®) Treated cell growth medium on the proliferation of human dermal fibroblast cells (HFF-1) for the assessment of skin health promotion potential. An aliquot of HFF-1 cells and DMEM were divided into two parts, one was treated with the Biofield Energy Treatment and denoted as the treated test samples, while other part was coded as the untreated test samples. MTT assay was used to study the cellular proliferation, while cell growth rate was estimated under phase contrast microscope by monitoring the cell growth at 24, 48, and 72 hours interval and photomicrographs were captured. The results of HFF-1 cellular proliferation rate showed a significant (p≤0.001) increased by 80% in the Biofield Energy Treated DMEM, while positive controls, ascorbic acid (10 µM) and FBS (15% fetal bovine serum) showed increased cell proliferation by 21% and 44%, respectively. In addition, cell growth rate was evaluated at 24, 48, and 72 hours and the results were observed with a significant growth in the Biofield Energy Treated DMEM group compared with the Biofield Energy Treated HFF-1 cells. Thus, on the basis of the experimental results, it can be concluded that The Trivedi Effect®-Consciousness Energy Healing Treatment has the significant capacity to improve the cellular proliferation and cell growth rate of human skin cells in the Biofield Energy Treated media (DMEM) for improving the overall skin health. It can be used as a complementary and alternative approach with respect to the various skin-related disorders, anti-aging, wound healing, and many more.

The results of cellular growth and proliferation data showed a significant improvement in the cell growth that suggest the significance of Biofield Energy Treatment (Consciousness Energy Healing Treatment - The Trivedi Effect®) in the HFF-1 cell line and DMEM for skin health. Biofield Energy Healing might be used as an alternative method in order to improve the overall skin health and aging. The present study showed significant results in in vitro skin health models with respect to HFF-1 cells. The cellular proliferation rate using MTT assay showed a significantly (P≤0.001) improved by more than 80% after treatment with the Biofield Energy Healing (The Trivedi Effect®) Treatment on DMEM and HFF-1 cells. In addition to, the cell growth rate was significantly improved in the Biofield Energy Treated DMEM group at 24, 48, and 72 hours study period as compared with the baseline control group. However, the Biofield Energy Treated HFF-1 cells did not show any significant change in proliferation rate. Overall, the data concluded that the Biofield Energy Treatment (The Trivedi Effect®) has a significant potential with respect to the wound healing, antiaging, and skin regeneration activities. Overall, The Trivedi Effect® can be used as a Complementary and Alternative Medicine (CAM) against skin irregularities that are typically symptoms of a skin disorders such as eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing, etc.