Effect of The Biofield Energy Healing Treatment on the Pharmacokinetics of 25-Hydroxyvitamin D3 [25(OH)D3] in Rats After a Single Oral Dose of Vitamin D3

There are numerous scientific studies on vitamin D3 reported to have many important roles viz. in bone metabolism, osteoporosis, immunity, and useful in several diseases, e.g., cancers, cardio-vascular, and neurodegenerative diseases. Surprisingly, there is little information available about the concentration of 25-hydroxyvitamin D3 in the blood, which is the best indicator of vitamin D3 status after its oral absorption. However, the biological activity of vit-D3 is mediated via the formation of the active metabolite, 1-α, 25-dihydroxyvitamin D3. There are several factors which affect its absorption and first-pass metabolism that lead to have very low plasma concentrations of both the metabolites (25-hydroxyvitamin D3 and 1-α, 25-dihydroxyvitamin D3) following an oral administration. Therefore, the present study was performed to determine the effects of The Trivedi Effect®-Energy of Consciousness Healing Treatment on vit-D3 and rats through the measurement of plasma 25-hydroxyvitamin D3 concentrations after the oral administration of vit-D3 in rats. The test item, vit-D3 was divided into two parts. One part was denoted as the control (without Biofield Energy Healing Treatment), while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Alice Branton. Additionally, one group of animals also received Consciousness Energy Healing Treatment per se by the Healer under similar conditions. Vitamin D3 oral formulations were administrated by oral gavage at a dose of 500 µg per kg in groups viz. G1 (untreated Vitamin D3), G2 (Biofield Treated Vitamin D3) and G3 (Biofield Treated animals received untreated Vitamin D3) group. The Biofield Energy Healing Treatment significantly altered the relative oral exposure (AUC0–t) of 25-hydroxyvitamin D3 by 54.62% in G2 group compared to the control group, G1. The Biofield Energy Treatment also altered plasma peak concentration (Cmax) of 25-hydroxyvitamin D3 by 53.8% and 7.6% in G2 and G3 groups, respectively compared to the control group. After oral administration, plasma concentrations of 25-hydroxyvitamin D3 in control group (G1) declined with slow excretion rate and the elimination half-life (T1/2) was 100.59 hours. The oral elimination half-life (T1/2) of 25-hydroxyvitamin D3 in G2 and G3 were 69.09 hours and 55.76 hours, respectively. The mean residence time (MRTlast) and elimination rate constant (Kel) remained unaltered in all three groups. The Trivedi Effect®-Energy of Consciousness Healing Treatment could be an innovative strategy which opens new avenues to overcome poorly absorbed pharmaceuticals/nutraceuticals/herbal extracts and can improve the therapeutic performance of orally active molecules.

The Trivedi Effect® -Energy of Consciousness Healing Treatment significantly altered the relative oral exposure (AUC0–t) of 25-hydroxyvitamin D3 by 54.62% in G2 group compared to the control group, G1. The Biofield Energy Treatment also altered plasma peak concentration (Cmax) of 25-hydroxyvitamin D3 by 53.8% and 7.6% in G2 and G3 groups, respectively as compared to the control group. After oral administration, plasma concentrations of 25- hydroxyvitamin D3 in the control group (G1) declined with slow excretion and the elimination half-life (T1/2) was 100.59 hours. The oral elimination half-life (T1/2) of 25-hydroxyvitamin D3 in the Biofield Energy Treatment group (G2) and Biofield Energy Treated rat group (G3) were 69.09 hours and 55.76 hours, respectively. The mean residence time (MRTlast) and elimination rate constant (Kel) were unaltered in all three groups. These data demonstrates altered plasma exposure and peak plasma levels of 25-hydroxyvitamin D3 in rats which might be translated into altered in vivo biological activity of vitamin D3. An alteration of oral exposure of 25- hydroxyvitamin D3 relative might be due to the alteration its physicochemical properties and thermal properties by Biofield Energy Treatment. Hence, The Trivedi Effect ® - Energy of Consciousness Healing Treatment is considered as an innovative strategy which opens new opportunities in order to improve the poorly absorbed drug/nutraceuticals/herbal extracts that can improve the therapeutic performance of orally active molecules. Overall, the data suggest that vitamin D3 level can be maintained for longer period of time which can be helpful to convert slowly into specific hydroxylated forms of vitamin D3. As a result, this treatment might be beneficial to the different groups of cardiac and kidney transplant patients, hip fracture patients, osteoporotic patients, hyperparathyroidism and cancer patient, neurodegenerative and ischemic heart patients.