Effects of Vitamin D3 on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Biofield Energy Healing Treatment

Silently progressing metabolic bone disorders are widely increased that affects the quality of life as one of the key health issues today. The aim of the study is to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Thomas Charles Slade and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone health biomarkers were studied. The cell viability (˃84%) using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 218.6% (100 µg/mL), 263.9% (10 µg/mL), and 135.7% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 147.6%, 153.1%, and 240.8% at 10, 50, and 100 µg/mL, respectively in the UT-DMEM+BT-TI group, while 150.8% and 212.9% at 50 and 100 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 104.6% and 185.9% in UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL, while 119%, 42.6%, and 102.3% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Overall, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Based on the study outcomes, it was found that the Biofield Energy Healing based vitamin D3 and DMEM showed a significant improved bone health parameters in MG-63 cell line. The test samples using MTT assay showed a significant improved cell viability with more than 84% in all the test samples at various concentrations. Additional, ALP data showed that the Biofield Energy Treated test samples in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups were reported with improved ALP by 218.6% (100 µg/mL), 263.9% (10 µg/mL), and 135.7% (100 µg/mL) respectively, as compared with the untreated group. Collagen level was significantly increased by 147.6%, 153.1%, and 240.8% at 10, 50, and 100 µg/mL, respectively in UT-DMEM+BT-TI while 138.2%, 138.4%, and 122.4% at 10, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, the level of collagen was increased by 150.8% and 212.9% at 50 and 100 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test group. Similarly, the bone mineralization percent was significantly increased by 104.6% and 185.9% in UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL, while 119%, 42.6%, and 102.3% increase was reported at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated test group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoma, rickets, low bone density and osteoporosis, osteomalacia, Paget’s disease of bone, bone and/or joint pain, osteogenesis imperfecta, increased frequency of fractures, deformed bones, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Ulcerative Colitis, Asthma, Alzheimer’s Disease, Graves’ Disease, Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Multiple Sclerosis, Aplastic Anemia, Hepatitis, Dermatitis, Dermatomyositis, Diabetes, Myasthenia Gravis, Irritable Bowel Syndrome, Parkinson’s Disease, Atherosclerosis, Diverticulitis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health and quality of life.