Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment

Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p≤0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line.

Scientific data suggests that an extensive aging could be prevented, while natural might be genetically determined. The Biofield Energy Healing might be an alternative method to improve the skin health and aging. The present study showed that the experimental in vitro skin health in HFF-1 cell model, which elucidate the significant role of Biofield Energy Healing (The Trivedi Effect®) Treatment on DMEM and HFF-1 cells in cellular proliferation along with an improved collagen level. The cellular proliferation rate using MTT assay was significantly increased (p≤0.001) by 85% in the Biofield Energy Treated DMEM group as compared with the baseline control group. Additionally, a significantly (p≤0.01) increased the collagen level by 44.2% was reported in the Biofield Energy Treated DMEM group as compared with the baseline control group. However, the Biofield Energy Treated HFF-1 cells did not show any significant change in proliferation rate and collagen level. Hence, it can be concluded that the Biofield Energy Treatment (The Trivedi Effect®) has the potential to have significant action as wound healing, antiaging, and skin regeneration.

Overall, the Biofield Energy Treated HFF-1 cells and DMEM can be used as a Complementary and Alternative Medicine (CAM) against skin irregularities that are typically symptoms of a skin disorders such as eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing

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