Evaluation of Immunomodulatory Properties After Oral Administration of Biofield Energy Treated Test Formulation in Sprague Dawley Rats: Influence of the Trivedi Effect®

A new proprietary herbomineral formulation was formulated, consisting of an essential ingredients viz. an herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of this study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed in this experiment. The humoral immune response data showed the secondary antibody titre was significantly increased by 112.50% and 87.50% in the Biofield Energy Treated test formulation group (G4) and untreated test formulation group (G5) compared to the disease control group (G2). The results of delayed type hypersensitivity showed significant increased the paw volume by 111.76%, 51.45%, 100%, and 64.80% in the G4, G5, Biofield Energy Treatment group per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7) groups, respectively compared to the G2 group. The platelet count was significantly increased by 37.72%, 8.69%, 16.30% and 33.11% in the G4, G5, G6, and G7 groups, respectively compared to the G2 group. The level of blood urea nitrogen was significantly decreased by 14.01% in the G4 group compared to the disease control. Moreover, the concentration of uric acid was significantly reduced by 28.97% in the G7 group compared to the G2 group. Animal weight parameters suggested that there were no treatment-related changes in any group. Organ to body weight ratio, feed and water intake data described that the Biofield Energy Treated test formulation was found to be safe without any side-effect during the course of the experiment. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Based on the current findings, it can be concluded that the humoral immune response, cell mediated immune response, platelet counts and others supportive biochemical parameters related to immunomodulation were significantly improved in the Biofield Energy Treated test formulation group (G4). The humoral immune response data showed the secondary response of mean hemagglutination (HA) antibody titre was significantly elevated by 112.50% in the Biofield Energy Treated test formulation (G4) compared to the G2 group. Further, a delayed type hypersensitivity (DTH) response was significantly increased by 111.76%, 51.45%, 100%, and 64.80% in the Biofield Energy Treated test formulation, untreated test formulation, Biofield Energy Treatment group per se at day -15 and Biofield Energy Treated test formulation at day -15, respectively compared to the disease control group (G2). The platelet count was significantly increased by 37.72% and blood urea nitrogen was significantly decreased by 14.01% in the Biofield Energy Treated test formulation group (G4) compared to the disease control group (G2). However, no treatment-related changes were observed in any group with respect to the body weight, feed intake, and water intake data in the Biofield Energy Treated test formulation and Biofield Energy Treatment per se group during the course of the experiment. Therefore, The Trivedi Effect®-Biofield Energy Healing Treatment administered remotely by the seven Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties without any toxic effect to the animals throughout the exposure period. Thus, the Biofield Energy Treated test formulation and Biofield Energy Treatment per se in male rats showed an effective anti-inflammatory and immunomodulatory action, and it can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.