Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation: Effect of Biofield Energy Healing Treatment

The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the formulation when applied to splenocyte cells isolated from mice spleen. The formulation was divided into two parts; one was the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The test formulation was evaluated to find the expression of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β along with non-cytotoxic concentrations by MTT assay. The splenocytes were given the Biofield Energy Treated and untreated sample at concentrations range (0.00001053 to 10.53 µg/mL) for 48 hours and was reported with safe concentration up to 1.053 µg/mL with percentage viability range from 76.7% to 109.2% in both samples. Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated formulation. The expression of TNF-α was significantly inhibited in the Biofield Treated formulation at 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively compared with the untreated formulation. The rest of the tested concentrations of the Biofield Treated formulation showed an increase in TNF-α expression at 0.00001053, 0.0001053, and 0.001053 µg/mL by 7.26%, 8.50%, and 8.50%, respectively compared to the vehicle control group. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppression activity at 0.01053 µg/mL by 18.47% (p≤0.001) compared to the untreated formulation. MIP-1α expression was reported as 628.94 ± 13.0 pg/mL in the untreated formulation, while it decreased to 512.74 ± 1.9 pg/mL in the Biofield Treated formulation at 0.01053 µg/mL. In addition, the IL-1β secretion was also significantly inhibited by the Biofield Treated formulation at concentrations 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. Overall, The Trivedi Effect® significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the treated formulation, which can be better utilized in organ transplants, anti-aging, stress management, autoimmune disorders, and inflammatory disorders, etc. to modulate the immune system by improving overall health.

The in vitro results of the Biofield Energy Treated and untreated test formulations showed significant modulatory effects on splenocyte cells isolated from mice with respect to the suppression of tested cytokines. All the tested cytokines (TNF-α, MIP-1α, and IL-1β) were reported with immunosuppressive activity with respect to the vehicle control group, while the Biofield Energy Treatment further potentiated the immunomodulation as compared with the untreated test formulation. The results of in vitro splenocyte cells’ viability assay suggest that most of the tested concentration of the Biofield Treated and untreated test formulations showed improved cell viability, except at the higher concentration of 10.53 µg/mL. The cell viability after the Biofield Energy Treatment significantly improved with the Biofield Treated test formulation at concentrations 0.0001053, 0.001053, and 1.053 µg/mL with respect to the untreated test formulation. The results of the cytokines estimation revealed that the Biofield Treated group suppressed the TNF-α, MIP-1α and IL-1β. TNF-α levels, which were significantly inhibited at three tested concentrations of 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively in the Biofield Treated test formulation as compared with the untreated test formulation. In the case of MIP-1α, both the untreated and Biofield Treated test formulation showed significant suppression, while the Biofield Energy Treatment enhanced the immunosuppressive activity at the concentration 0.01053 µg/mL by 18.47% (p≤0.001) as compared to the untreated formulation. The values of MIP-1α reported in the case of the untreated formulation was 628.94 ± 13.0 pg/mL, while it was decreased to 512.74 ± 1.9 pg/mL in the Biofield Energy Treated test formulation. Similarly, significant inhibition (p≤0.001) of IL-1β secretion was reported in the Biofield Treated test formulation at concentration 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation.

On the basis of the above estimation, it can be concluded that after receiving the Biofield Energy Healing Treatment (The Trivedi Effect®), administered remotely by the seven Biofield Energy Healers to the new herbomineral formulation significantly inhibited the activity of pro-inflammatory cytokines that might prevent the over-activation of the immune system. The Biofield Energy Treated test formulation can be a better complementary and alternative medicine to prevent the immune-mediated tissue damage in cases of organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis (MS), Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease and stress etc. with a safe therapeutic index to improve overall health and quality of life. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.