Evaluation of Skin Protective Effect Using in Vitro Cell Line Models of Biofield Energy-Consciousness Energy Healing Treated Herbomineral Formula

Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.

The experimental data exhibited significantly improved cell viability evaluated using MTT assay. The data showed more than 70% cells were viable in all the tested concentrations such as HFF-1 (1.25 to 10 µg/mL), HaCaT (0.625 to 5 µg/mL), and B16-F10 (10 to 40 µg/mL). BrdU assay indicated 43.63% and 10.97% increase in the cellular proliferation in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. The collagen level was increased by 23.56% and 13.36% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation group respectively, at the concentration of 1.25 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly increased by 8.34% in the BT-DMEM + BT-Test formulation at 2.5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was significantly increased by 2.60% in the BT-DMEM + UT-Test formulation group at 0.625 µg/mL compared to the UT-DMEM + UT-Test formulation group. Melanin level was reduced by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the UT-DMEM + UT-Test formulation group at 0.125 µg/mL. Anti-wrinkling effects with respect to UV-B showed 4.17% and 5.88% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation group respectively, compared to the UT-DMEM + UT-Test formulation group. The wound healing scratch assay indicated a significant migration of fibroblast and keratinocytes cells with increased covered area up to 10.50% and 8.30% in HFF-1 and HaCaT cells respectively after treatment with the Biofield Energy based herbomineral test formulation. Altogether, the Biofield Energy Treated Test formulation (The Trivedi Effect®-Consciousness Energy Healing Treatment) and DMEM have shown significant skin protection action, which suggests its use against wrinkling, aging, skin whitening, and wound healing. Therefore, test formulation could be suitable for prevention and improvement of various skin related disorders as a novel herbal cosmetics.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.