Functional Modification of Bone Tissues After Treatment with the Biofield Energy Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63)

The potential of Consciousness Energy Treated vitamin D3 and DMEM in human bone osteosarcoma cells (MG-63) was investigated. The Test Items (TI), were separated into two parts. One part of each sample was received Biofield Energy Treatment by Joy Angevin Balmer and defined as Biofield Treated (BT) samples, while other parts of each sample were denoted as Untreated Test Items (UT). The cell viability results revealed test samples were found as safe upto 100 µg/mL. ALP was significantly increased by 84.62% in BT-DMEM + BT-TI at 0.1 µg/mL, while increased by 118.24% and 241.62% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 1 µg/mL than UT-DMEM + UT-TI. Moreover, ALP was significantly elevated by 43.56% and 192.08% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 10 µg/mL than untreated. Collagen was significantly increased by 112.24%, 83.68%, and 142.88% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 0.1 µg/mL than untreated. Further, collagen was significantly elevated by 75.39%, 106.15%, and 113.87% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI at 1 µg/mL than UT-DMEM + UT-TI. Besides, bone mineralization was remarkably increased by 106.2% and 95.20% at 100 µg/mL in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively than untreated. Overall, Biofield Energy Treated vitamin D3 was significantly improved bone health parameters and could be able to fight against various bone-related disorders (osteoporosis, rickets, low bone density, osteogenesis imperfecta), autoimmune and inflammatory diseases, stress, and anti-aging improving overall health.

The MTT cell viability assay results exhibited more than 77% cells were viable, which supported that the test samples were found as safe and nontoxic in all the tested concentrations. The UT-DMEM + BT-Test item group exhibited 118.24% and 43.56% increased the level of ALP at 1 and 10 µg/mL, respectively as compared to the untreated group. Moreover, the BT-DMEM + UT-Test item group increased of ALP by 241.62% and 192.08% at 1 and 10 µg/mL, respectively while BT-DMEM + BT-TI showed 84.62% at 0.1 µg/mL compared to the untreated group. Collagen was significantly increased by 112.24%, 83.68%, and 142.88% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1 µg/mL, while increased by 75.39%, 106.15%, and 113.87% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was remarkably increased by 23.94%, 106.2%, and 95.20% at 100 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively compared to the untreated group.

Thus, the Biofield Energy Treated test samples (The Trivedi Effect®) demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable for the development of an alternative and more effective supplement for vitamin D3 deficiency, which could be useful for the management of various bone related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, etc. Besides, it can also be utilized in organ transplants (kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, inflammatory diseases, anti-inflammatory, anti-stress, anti-arthritic, anti-osteoporosis, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic actions stress management and prevention, and anti-aging by improving overall health, Parkinson’s Disease and stress etc. to modulate the immune system by improving overall health.