Immunological Effects of Biofield Energy Healing (The Trivedi Effect®) Based Novel Herbomineral Formulation After Oral Administration in Male Sprague Dawley Rats

Herbomineral formulations have been used and accepted all around the world owing to its medicinal and therapeutic importance. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) Treatment on the new proprietary herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The Biofield Energy Treated and untreated test formulation was evaluated using experimental parameters such as humoral immune response (primary and secondary antibody titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry were studies. Humoral immune data showed the primary and secondary antibody titre values were significantly (p≤0.05) increased by 105.88% and 260%, respectively in the Biofield Energy Treated Test formulation (G4) group with respect to the disease control group (G2). The delayed type hypersensitivity results showed a significant increased paw edema by 32.43% and 53.33% at 24 and 48 hours, respectively in the Biofield Energy Treated Test formulation (G4) group, while untreated test formulation group (G5) animals showed 13.33% increase in the paw edema (at 48 hour) compared with the G2 group. Hematological analysis showed a significant increase in the red blood cell count, while the platelet count was significantly increased by 27.78% in Biofield Energy Treated Test formulation (G4) group as compared with the G2 group. Biochemical analysis exhibited an increased level of calcium and phosphorus by 1.58% and 12.65%, respectively while serum uric acid was significantly decreased by 36.66% in the G4 group compared with the G2 group. In addition, animal weight parameters like body weight, relative organ weight, histopathology, water and feed intake data suggest that the Biofield Energy Treated herbomineral formulation was found safe, non-toxic, throughout the exposure period. In conclusion, the The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation would be the powerful immunomodulatory product, which can be useful to modulate the immunity against many infectious diseases. Overall, experimental data suggests that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

In conclusion, the data obtained from the present study revealed that the Biofield Energy Treated (The Trivedi Effect®) ashwagandha based herbomineral formulation possesses significant immunomodulatory activity compared with the untreated test formulation. Humoral immune response results suggest that the primary and secondary antibody titre values were significantly increased by 105.88% and 260% (p≤0.01), respectively in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. However, untreated test formulation (G5) showed 64% increase in the secondary antibody titre and decrease in primary antibody titre compared with the G2 group. Delayed-type hypersensitivity (DTH) response in rats showed significant increase in the paw thickness by 32.43% and 53.33% (p≤0.05) at 24 and 48 hours, respectively in the G4 group compared with the disease control (G2) group. However, the percentage increase in the DTH response was higher in Biofield Energy Treated test formulation compared with the untreated test formulation (13.33%). Hematological study showed an increase in the RBC count, while the platelet count was significantly increased by 27.78% in the Biofield Energy Treated test formulation (G4) group compared with the disease control group (G2). Biochemical analysis showed significant decrease in the serum uric acid level by 36.66%, while the levels of magnesium, calcium, phosphorus, and potassium ions were increase in the Biofield Energy Treated test formulation (G4) group compared with the disease control group. In addition, the animal weight parameters such as body weight, relative organ weight, water intake, and feed intake were significantly correlated with the histopathological findings, which suggest no significant change in the Biofield Energy Treated test formulation group (G4). It mentioned that the test formulation was safe, non-toxic, and did not have any injurious effect.

Therefore, the current findings concluded that The Trivedi Effect®-Biofield Energy Healing administered remotely by the twenty Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties without any side effect, which can be used as a herbomineral product to improve the overall health. Thus, the Biofield Energy Treated test formulation may act as an effective anti-inflammatory and immunomodulatory product, and it can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.