Impact of Biofield Energy Healing Treated Vitamin D3 on Human Osteoblast Cell Line (MG-63) for Bone Health

Bone disorders are largely associated with increased morbidity, mortality, and substantial economic and health costs. Vitamin D play an important role for calcium absorption and bone mineralization, which can improve the patients' quality of life. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by William Dean Plikerd and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 346.4% (at 50 µg/mL), 375.3% (at 100 µg/mL), and 343.2% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI at 10 and 50 µg/mL, respectively while 399.3% (10 µg/mL) and 200% (0.1 µg/mL) increased collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively. Moreover, the percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups by 416.9% (10 µg/mL), 460.7% (0.1 µg/mL), and 441.7% (10 µg/mL) respectively as compared with the untreated test item and DMEM group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

The experimental results showed a significant effect of Biofield Energy Healing Treatment on vitamin D3 and DMEM for bone health in MG-63 cell line. Cell viability test using MTT assay showed a significant improved the cell viability with more than 80% cell viability among different test groups, while Biofield Energy Treated test vitamin D3 and DMEM also improved the cell viability as compared with the untreated group. Cell viability test using MTT assay showed that the test items were safe and nontoxic in all the tested concentrations. After Biofield Energy Healing Treatment, the concentration of ALP was significantly increased by 343.2% and 375.3% in the BT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 100 µg/mL, while 346.4% increased ALP was reported in the UT-DMEM+BT-TI group at 50 µg/mL as compared to the untreated group. In addition, the level of collagen was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI group at 10 and 50 µg/mL, respectively, while 399.3% (10 µg/mL) and 200% (0.1 µg/mL) increased collagen in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Similarly, the percent of bone mineralization was significantly increased by 416.9% at 10 µg/mL in the UT-DMEM+BT-TI group, while BT-DMEM+UT-TI group showed increased bone mineralization by 49.4%, 23.1%, and 20.6% at 10, 50, and 100 µg/mL, respectively as compared with the untreated group. In addition, bone mineralization in MG-63 cells was increased by 441.7% in the BT-DMEM+BT-TI group at 10 µg/mL, 460.7% at 0.1 µg/mL in BT-DMEM+UT-TI, and 416.9% at 10 µg/mL in UT-DMEM+BT-TI as compared with the untreated group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples showed a significant impact on bone health parameters viz. collagen, calcium and ALP, which play a vital role in maintaining bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-stress, anti-aging, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, cell cycling and proliferation, hormonal balance, neurotransmission, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, stress etc. with a safe therapeutic index to improve overall health and quality of life.