Impact of Biofield Energy Treated (The Trivedi Effect®) Herbomineral Formulation on the Immune Biomarkers and Blood Related Parameters of Female Sprague Dawley Rats

The aim of the present study was to evaluate the immunomodulatory potential of Biofield Energy Treatment (The Trivedi Effect®) on a new proprietary compound composed of herbs and minerals (herbomineral formulation). The test formulation contains the mixture of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. The immunomodulatory parameters viz. humoral immune response (IgG and IgM), cellular immune response (CD4+/CD8+), hematological parameters, lipid profile, hepatic enzymes, and sex hormones (progesterone and estrogen) in female Sprague Dawley rats were analyzed. The results of humoral immune response data showed decreased levels of IgG and IgM by 80.54% and 78.94% respectively, in the Biofield Energy Treated test formulation group (G3) compared with the disease control group (G2). Estimation of cellular immune response data revealed a significant (p≤0.01) increase in the ratio of CD4+/CD8+ by 161.71% in the G3 group, while it was increased by 100.50% in the untreated test formulation group (G4) compared with the G2 group. Thus, the humoral and cellular immune responses were significantly altered in the G3 as compared with the G4 group. The TLC and neutrophils counts were significantly increased by 11.34% and 1.34% respectively, in the G3 compared with the G2 group. Lipid profile data suggested that the Biofield Energy Treated test formulation showed improvement in the tested parameters compared with the untreated test formulation group. However, significant improvement was found in the tested hepatic biomarkers i.e. decreased levels of the SGOT, SGPT, and CK-MB by 14.28%, 8.54%, and 20.79% in the G3 group compared with the G2 group. The levels of total protein, alkaline phosphatase, albumin, and globulin were increased by 11.93%, 1.24%, 6.10%, and 20.74% respectively, in the G3 compared with the G2 group. Consequently, the levels of progesterone and estrogen were significantly increased by 199.86% and 50.19%, respectively in G3 with respect to the G2 group. In conclusion, overall data suggest that The Trivedi Effect®-Biofield Energy Healing Treatment on the herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management, and anti-aging by improving overall health.

Based on the current study findings, the Biofield Energy Healing Treatment on the herbomineral formulation showed a significant increase in the humoral and cellular immune responses. The immunological profile suggested that the levels of IgG and IgM were significantly altered in the Biofield Energy Treated test formulation group compared with the disease control group. Further, the CD4+/CD8+ ratio was significantly (p≤0.01) increased by 161.71% in the Biofield Energy Treated test formulation group compared with the disease control group. The increased level of the CD4+/CD8+ ratio is directly related to improved immunity against many diseases. The levels of TLC and neutrophils were significantly increased by 11.34% and 1.34% respectively, in the Biofield Energy Treated test formulation group compared with the disease control group. Additionally, the lipid profile data suggest the levels of total cholesterol, triglycerides, HDL, LDL, and VLDL were significantly altered in the Biofield Energy Treated test formulation. Additionally, the hepatic profile was significantly altered in terms of total protein, alkaline phosphatase, albumin, and globulin level in the Biofield Energy Treated test formulation group, while the hepatic biomarkers like SGOT, SGPT, and cardiac biomarker, CK-MB were significantly decreased by 14.29%, 8.54%, and 20.79%, respectively with respect to the disease control group. Further, the level of sex hormones, i. e. progesterone and estrogen were significantly increased by 199.86% and 50.19% in the Biofield Energy Treated test formulation group, while the untreated test formulation showed decreased level of progesterone and estrogen by 86.34% and 6.66%, respectively compared with the disease control group. Therefore, the Trivedi Effect® Biofield Energy Healing administered remotely by the eighteen Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties in terms of improved cellular immune response, hematological profile, hepatic biomarkers, and increased sex hormone levels.

Overall, the Biofield Energy Treated test formulation can be used as a CAM with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.