Improved Structure and Development of Bone using Biofield Energy Enriched Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63)

The present study aimed to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters in MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. Both the samples received Consciousness Energy Healing Treatment by Maria Isabel Aguilar Tiraboschi and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 70% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 191.7% (0.1µg/mL), 127.8% (at 1µg/mL), and 136.1% (at 10µg/mL) in BT-DMEM+UT-TI, BT-DMEM+BT-TI, and UT-DMEM+BT-TI groups respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 267.9%, 128.9%, and 91.8% at 1, 10, and 50µg/mL, respectively in UT-DMEM+BT-TI group, while 169.6% at 1µg/mL in BT-DMEM+UT-TI group respectively as compared with the untreated group. Besides, BT-DMEM+BT-TI group showed an increased collagen value by 155.4% and 100% at 1 and 10 µg/mL respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 139.8%, 91%, and 44.7% at 0.1, 10, and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 126.7%, 79.3%, and 67.5% at 0.1, 10, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 31.3%, 33.2%, and 152.5% at 0.1, 10, and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

The cell viability using MTT assay results showed a significant improved viability with more than 70% among the tested groups, which suggested that the test samples are safe and nontoxic. Bone health parameters such as the level of ALP was increased by 136.1% at 10µg/mL in the UT-DMEM+BT-TI, 191.7% at 0.1µg/mL in BT-DMEM+UT-TI, while 127.8% at 1µg/mL in the BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. The level of collagen was significantly increased by 267.9%, 128.9%, and 91.8% at 1, 10 and 50µg/mL, respectively in the UT-DMEM+BT-TI, while 169.6% and 56.7% at 1 and 10µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, collagen level was increased by 155.4% and 100% at 1 and 10µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 139.8%, 91%, and 44.7% at 0.1, 10 and 100µg/mL, respectively in the UT-DMEM+BT-TI group, while 126.7%, 79.3%, and 67.5% at 0.1, 10, and 100µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 31.3%, 33.2%, and 152.5% at 0.1, 10, and 100µg/mL, respectively as compared with the untreated group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influences cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Ulcerative Colitis, Alzheimer’s Disease, Dermatitis, Irritable Bowel Syndrome, Asthma, Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Multiple Sclerosis, Aplastic Anemia, Hepatitis, Diverticulitis, Graves’ Disease, Dermatomyositis, Diabetes, Myasthenia Gravis, Parkinson’s Disease, Atherosclerosis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health, and quality of life.