In Vitro Effects of Biofield Energy Treated Vitamin D3 Supplemen-tation on Bone Formation by Osteoblast Cell Line (MG-63)

Inadequate intake of vitamin D leads to hormonal imbalance, aging, decreased calcium absorption, and bone loss. Present study aimed to evaluate potential of The Trivedi Effect®- Biofield Energy Healing Treatment on vitamin D3 as Test Item (TI) and DMEM to improve bone health in MG-63 cells. One part of each samples was received Consciousness Energy Healing Treatment by Dahryn Trivedi and those samples were labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as untreated TI (UT-TI). Cell viability assay (MTT) found test items were safe and nontoxic in the tested concentrations. ALP was considerably improved by 753.3%, 1173.3%, and 424.4% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared to untreated. Collagen was significantly increased by 454.7% and 96.2% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 1 µg/mL, while 202.4% (at 50 µg/mL) increased collagen in UT-DMEM+BT-TI compared to untreated. Moreover, percent of bone mineralization was significantly increased in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI by 301.4% (at 50 µg/mL), 143.3% (at 50 µg/mL), and 178.0% (at 100 µg/mL) respectively, compared to untreated. Thus, Biofield Energy Treated vitamin D3 and Biofield Energy Treated DMEM were found safe and to have remarkably improved the bone health parameters, which could be a powerful alternative nutraceutical supplement to combat against various bone-related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

The present in vitro experiment showed a significant effect of the Biofield Energy Treatment on vitamin D3 and DMEM for various bone health parameters. MTT assay was used for cell viability at the various test concentrations that showed a significant improved cell viability greater than 80%, while Biofield Energy Treated test items improved the cell viability as compared with the untreated group. ALP level was significantly increased by 753.3%, 1173.3%, and 424.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 0.1 µg/mL, compared to the untreated group. In addition to, the collagen level was significantly increased by 454.7% and 96.2% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 1 µg/mL, while 202.4% increased in collagen was also reported at 50 µg/mL in the UT-DMEM+BT-TI compared to the untreated group. Likewise, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 301.4% and 143.3% respectively, at 50 µg/mL, while 178.0% increased in bone mineralization in BT-DMEM+BT-TI group at 100 µg/mL as compared to the untreated test item and DMEM group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples showed a significant impact on various bone health parameters viz. collagen, calcium, and ALP, which play a vital role in maintaining bone-related disorders. Thus, Biofield Treated vitamin D3 might be an alternative nutritional supplement, which could be useful for the management of various bone-related disorders viz. chondrodystrophia fetalis, rickets, low bone density, osteoporosis, osteogenesis imperfecta, Paget’s disease, osteomalacia, bone fractures, deformed bones, osteoma, and other bone disorders. Biofield Treated vitamin D3 can be useful as anti-stress, anti-aging, anti-inflammatory, wound healing, anti-cancer, anti-psychotic and anti-fibrotic roles. It also influences normal cell growth, cell-to-cell communication, cell differentiation, cell cycling and proliferation, hormonal balance, and neurotransmission. Moreover, it can be used in organ transplants like liver, heart, and kidney transplants and various immune-related disease conditions such as Myasthenia Gravis, Parkinson’s Disease, Ulcerative Colitis, Dermatitis, Alzheimer’s Disease, Diverticulitis, Atherosclerosis, Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Pernicious Anemia, Aplastic Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Hepatitis, Irritable Bowel Syndrome, etc. with a safe therapeutic index to improve overall health and Quality of Life (QoL).