Influence of the Biofield Energy Treated Vitamin D3 on Human Osteoblast-Like Cells

The quality of life after rapid bone loss among individuals is one of the key health issues today, which might be due to hormonal factors and lack of proper nutrition that lead to an increased risk of fractures. The experimental data suggested a significant potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters. Vitamin D3 and DMEM medium as test items (TI), which were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. The cell viability (˃92%) using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 137.8%, 126.2%, and 102.5% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100µg/mL. Collagen content was significantly increased by 141.5% and 307.3% in the UT-DMEM+BT-TI and BT-DMEM+BT-TI group, respectively at 1µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 293.7% and 76.3% at 0.1 and 1µg/mL, respectively in UT-DMEM+BT-TI, while 122.9% (at 1µg/mL) and 165.1% (at 0.1µg/mL) in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders (such as osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures), along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Based on the study outcomes, test samples using MTT assay was assessed for cell viability and data showed more than 92% among the tested groups, which suggest significant cell growth, proliferation, and morphological effects. ALP data showed that the Biofield Energy Treated test samples by 65.9%, and 137.8% at 50 and 100µg/mL, respectively in UT-DMEM+BT-TI, while BT-DMEM+UT-TI group showed a significant increased ALP level by 70.4% and 126.2% at 50 and 100µg/mL, respectively. In addition, BT-DMEM+BT-TI group showed a significant increased ALP level by 56.9% and 102.5% at 50 and 100µg/mL, respectively as compared with the untreated group. Collagen level was significantly increased by 141.5%, 56.5%, and 38.9% at 1, 10, and 50µg/mL, respectively in UT-DMEM+BT-TI, while 307.3%, 80.1%, and 76.7% at 1, 10, and 50µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Similarly, the bone mineralization percent was significantly increased by 293.7% and 76.3% at 0.1 and 1µg/mL, respectively in UT-DMEM+BT-TI group, while 122.9% (at 1µg/mL) and 165.1% (at 0.1µg/mL) in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated test group. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoma, rickets, low bone density and osteoporosis, osteomalacia, Paget’s disease of bone, bone and/or joint pain, osteogenesis imperfecta, increased frequency of fractures, deformed bones, chondrodystrophia fetalis, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), hormonal imbalance, aging, and various immune related disease conditions such as Ulcerative Colitis, Alzheimer’s Disease, Dermatitis, Irritable Bowel Syndrome, Asthma, Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Multiple Sclerosis, Aplastic Anemia, Hepatitis, Diverticulitis, Graves’ Disease, Dermatomyositis, Diabetes, Myasthenia Gravis, Parkinson’s Disease, Atherosclerosis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health, and quality of life.