Investigation of Biofield Treated Vitamin D3 to Improve Immunity of Bone Cells in MG-63 Cell Line.

The study was aimed to evaluate the effect of Consciousness Energy Healing-based vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63). Test items (vitamin D3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Inthirani Arul and those samples were denoted as Biofield Treated (BT) samples, while other parts of each sample were referred as untreated test items (UT). Cell viability revealed that test samples were found as safe in tested concentrations. ALP was significantly increased by 260% in BT-DMEM + BT-TI group at 0.1 µg/mL compared to UT-DMEM + UT-TI group. Moreover, collagen level was significantly increased by 102.94% and 52.94% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 0.1µg/mL than UT-DMEM + UT-TI group. Further, collagen level was significantly increased by 114.94%, 114.03%, and 152.33% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1µg/mL than untreated. Besides, percent of bone mineralization was significantly enhanced by 82.95% and 119.55% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 10µg/mL than untreated. Moreover, it was significantly increased by 166.44% and 212.49% in BT-DMEM + UT-TI and BT-DMEM + BT-TI groups, respectively at 50µg/mL than untreated. It was also significantly increased by 104.44%, 100%, and 230.22% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100µg/mL than untreated. Among three combination regimens, the Biofield Treated vitamin D3 and DMEM group was comparatively more improved the bone-related parameters and might be useful against various bone-related disorders (rickets, low bone density, osteomalacia, osteoporosis, Paget’s disease, chondrodystrophia fetalis), stress management, anti-aging, autoimmune and inflammatory disorders near future.

Based on the cytotoxicity data it was observed that the tested test items were nontoxic at a selected concentrations, and were used further for the analysis of others bone-related parameters. Results showed that alkaline phosphatase (ALP) level was significantly (260%) increased in the Biofield Treated vitamin D3 and DMEM group as compared with the untreated group. Structural protein of bone like collagen was significantly increased (more than 100%) in all three groups even at lower concentration (1 µg/mL). Moreover, Biofield Treatment was also significantly increased (upto 230%) bone mineralization compared to the untreated group. In conclusion, the Biofield Energy Treated (The Trivedi Effect®) test samples demonstrated an impact on bone parameters like ALT, collagen, and bone mineralization. Therefore, it might be suitable for the development of an alternative supplement for vitamin D3 deficiency than naïve vitamin D3, for the management of various bone-related disorders viz. osteoporosis, rickets, osteomalacia, etc. Besides, as it also improved the structural protein (collagen) of connective tissues, could be utilized in organ transplants, autoimmune disorders (addison disease, rheumatoid arthritis, multiple sclerosis, myasthenia gravis, pernicious anemia, type 1 diabetes, crohn’s disease, etc.), inflammatory disorders (ulcerative colitis, irritable bowel syndrome, etc.), anti-stress, wound healing, anti-cancer, anti-aging, anti-psychotic, etc. to modulate the immune system by improving Quality of Life (QoL).