Modulation of Immune Biomarkers by Biofield Energy Healing Based Herbomineral Formulation in Male Sprague Dawley Rat: Potential Role of Energy of Consciousness

The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment based new proprietary herbomineral formulation and Energy Healing Treatment per se in male Sprague Dawley rats for immune biomarkers modulation. The test formulation was divided into two parts, one as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by under similar conditions. Humoral immune response data showed an increased level of IgG by 2.89%, 6.20%, and 6.20% in the Biofield Energy Treated test formulation group (G4), Biofield Energy Treatment group per se (G6), and Biofield Energy Treated test formulation at day -15 (G7), respectively as compared with the disease control group (G2). The ratio of CD4+/CD8+ was increased by 3.80%, 12.38%, and 16.19% in the G4, G6, and G7, respectively compared with the G2. Hematology analysis suggested an increased level of TLC and neutrophil by 9.64% and 1.48%, respectively in the G4 group, while G6 group showed an increase count of TLC, lymphocytes, and monocytes by 19.79%, 2.87%, and 15.54%, respectively compared with the G2. Biochemical study showed an increased concentration of glucose by 11.78%, 18.60%, and 56.84% in the G4, G6, and G7, respectively compared with G2. Total cholesterol was significantly decreased by 10.54% and 4.20% in the G4 and G7, respectively compared with G2. In contrast, the HDL level was increased by 11.69% and 1.06% in G6 and G7, respectively while LDL was decreased by 10.52% in G4 compared with the G2. SGOT, CK-MB, total protein, albumin, and globulin levels were decreased by 4.04%, 23.54%, 1.68%, 1.54%, and 1.80%, respectively in G4, while G6 group showed decreased level of SGOT, CK-MB, and A/G ratio by 2.08%, 16.23%, and 3.40%, respectively compared with G2. However, SGOT was significantly decreased (p≤0.01) by 18.88% in G7 compared with G2. The testosterone level was decreased by 76.67%, 18.43%, and 44.06% in G4, G6, and G7, respectively compared with G2. Antioxidant profile showed a decreased level of LPO by 59.63% in G7, while SOD and CAT levels were significantly altered in tested groups compared with G2. Biofield Energy may also be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

Based on the current findings and the results of the immune biomarkers assay after oral administration of herbomineral formulation exhibited significantly impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on animals and test formulation group, which showed a significant improved humoral and cellular immunity. In case of humoral immune response, the IgG level was significantly increased by 2.89%, 6.20%, and 6.20% in the Biofield Energy Treated test formulation (G4), Biofield Energy Treatment per se at day -15 (G6), and Biofield Energy Treated test formulation at day -15 (G7), respectively as compared with the disease control (G2). The ratio of CD4+/CD8+ as cellular immune response was increased in the G4, G6, and G7 groups by 3.80%, 12.38%, and 16.19%, respectively compared with the G2 group. This increase in CD4+/CD8+ ratio suggest strong immune strength after Biofield Energy Healing. Besides, a significant improved hematological profile such as TLC and neutrophil was increased by 9.64% and 1.48%, respectively in the G4 group compared with disease control group (G2). However, Biofield Energy Treatment group per se (G7) also showed a significant increased levels of TLC, lymphocytes, and monocytes by 19.79%, 2.87%, and 15.54%, respectively compared with the disease control (G2). Similarly, the Biofield Energy Treated test formulation (day -15) (G7) group showed an improved level of TLC, lymphocytes, eosinophils, and monocytes by 51.26%, 3.50%, 7.43%, and 36.55%, respectively compared with disease control group (G2). In biochemical estimation, the level of total cholesterol was significantly decreased in the Biofield Energy Treated test formulation and Biofield Energy Treated test formulation at day -15 by 10.54% and 4.20%, respectively compared with the disease control. Similarly, HDL level was increased by 11.69% in the Biofield Energy Treatment per se (day -15) group compared with the disease control group (G2). LDL level was significantly decreased by 10.52% in the Biofield Energy Treated test formulation (G4) compared with the disease control group (G2). Among hepatic biomarkers, SGOT, TP, A, and G levels were significantly decreased, while CK-MB level was decreased by 23.54% in the Biofield Energy Treated test formulation (G4) as compared with the disease control (G2). Besides, Biofield Energy Treatment per se group (G6) showed decrease in the values of SGOT, CK-MB, and A/G by 2.08%, 16.23%, and 3.40%, respectively compared with the disease control. The level of testosterone was significantly altered in the Biofield Energy Treated test formulation compared with the disease control group. Antioxidant assay suggests that the free radical, LPO was significantly decreased by 59.63% in the Biofield Energy Treated test formulation at day -15 (G7) compared with the disease control, while antioxidants such as SOD and catalases were significantly altered after Biofield Energy Treatment to the test formulation compared with the disease control group.

Overall, it can be concluded that the novel herbomineral formulation after treatment with the Trivedi Effect®-Biofield Energy Healing remotely by the seven Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties. Therefore, the Biofield Energy Treated test formulation may act as an effective anti-inflammatory and immunomodulatory product, and it can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.