Overall Skin Health Potential of the Biofield Energy Healing Based Herbomineral Formulation Using Various Skin Parameters

The aim of the present study was to evaluate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against various skin health parameters using three cell lines i.e., HFF-1, HaCaT, and B16-F10. The various study parameters viz. collagen, elastin, hyaluronic acid, melanin, cell viability against UV-B induced stress, and wound healing were evaluated. The test formulation and DMEM were divided into two parts. One part of the test formulation and one part of the DMEM received the Consciousness Energy Healing Treatment by Janice Patricia Kinney and were defined as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test samples. Cell viability using MTT assay showed more than 70% cells were viable in all the tested concentrations in three cells, indicating that the test formulation was safe and nontoxic. The collagen synthesis was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively in relation to the UT-DMEM + UT-Test formulation group. The Elastin level was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the untreated group. Hyaluronic acid at 0.63 µg/mL was increased significantly by 15.90% in the BT-DMEM + BT-Test formulation group compared to the untreated group. The level of melanin was reduced significantly by 9.25% and 7.26% in the BT-DMEM + BT-Test formulation group at 0.013 and 0.063 µg/mL, respectively in relation to the untreated group. Protection of skin cells after UV-B exposure data displayed that the cell viability was increased significantly by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing data exhibited significant wound closure and cell migration activities in the HFF-1 and HaCaT cells compared to the UT-DMEM + UT-Test formulation. Overall, the data suggests that the Biofield Energy Treated DMEM and test formulation demonstrated better responses compared to the untreated medium and test formulation with respect to the tested skin health parameters. Therefore, the Biofield Energy Healing and the Treated test formulation could be developed as an effective cosmetic product to protect and treat the various skin problems including infection, photosensitivity, erythema, contact dermatitis, seborrheic dermatitis, athlete's foot, psoriasis, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color, etc.

The study findings describe that the cell viability of the test formulation using MTT assay exhibited more than 70% cells were viable in all the tested concentrations, indicating that the test formulation was found to be safe and nontoxic. The collagen level was significantly (p≤0.001) increased by 22.42% and 17.48% in the UT-DMEM + BT-Test formulation group at 0.63 and 2.5 µg/mL, respectively with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly (p≤0.001) increased by 28.41% in the BT-DMEM + BT-Test formulation group at 5 µg/mL compared to the UT-DMEM + UT-Test formulation group. Moreover, the elastin level at 10 µg/mL was significantly (p≤0.001) increased by 24.12% and 117.65% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was increased significantly by 32.46% in the BT-DMEM + UT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. However, it was further increased by 15.90% and 17.67% in the BT-DMEM + BT-Test formulation group at 0.63 and 1.25 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Melanin level was significantly reduced by 9.25% in the BT-DMEM + BT-Test formulation group at 0.013 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The anti-wrinkle activity after challenged with UV-B, revealed that the percent cell viability was significantly increased by 17.88%, 20.10%, and 25.77% in the BT-DMEM + BT-Test formulation group at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the UT-DMEM + UT-Test formulation group. Wound healing results described that the cell coverage area was significantly improved in both the HFF-1 and all HaCaT cells compared to the untreated group. Altogether, the Consciousness Energy Healing Treated (The Trivedi Effect®) test formulation and DMEM showed significant protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Therefore, the Biofield Energy Healing and the Treated test formulation could be suitable for the development of herbal cosmetics, which would be useful for the management of wounds and various skin related disorders like contact dermatitis, pimples, cellulitis, impetigo, chickenpox, scabies, syringoma, rosacea, photosensitivity, urticaria, hives, warts, abscess, callus, acne, eczema, seborrheic dermatitis, athlete's foot, psoriasis, abscess, erythema, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color etc.