Photo-Protective Effect of Biofield Energy Healing (The Trivedi Effect®) Treatment Based Herbomineral Formulation Against Various Skin Health Parameters

The current study aimed to evaluate the effect of Biofield Energy Healing (The Trivedi Effect®) based herbomineral formulation on various skin health parameters. The test formulation consisted of minerals (zinc, selenate, and molybdenum), L-ascorbic acid, along with Centella asiatica extract, and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts, one part of each was treated with the Biofield Energy Treatment by Aksana Hancharuk and denoted as treated group, while other part was coded as the untreated groups. Skin parameters against various cell lines such as HFF-1, HaCaT and B16-F10 were examined with the combination of Biofield Treated (BT) and untreated test formulation (UT) with DMEM. MTT assay result showed that the test formulation was found to be safe and nontoxic at tested concentrations. Human fibroblast cell proliferation using BrdU method showed a significant increased cell proliferation by 91.51% and 201.14% in different Biofield Energy Treated groups. UT-DMEM + BT-Test formulation group showed an increased level of collagen by 64.58% (2.5 µg/mL) and 55.55% (1.25 µg/mL), respectively compared with the untreated group. Similarly, elastin synthesis was increased in UT-DMEM + BT-Test formulation group by 21.96% and 11.42% at concentration 10 and 5 µg/mL, respectively compared to the untreated group. However, hyaluronic acid level was increased by 14.02% and 6.57% at concentrations 1.25 and 0.625 µg/mL, respectively in BT-DMEM + BT-Test formulation group compared with the untreated group. Moreover, inhibition of melanin synthesis was observed by 12.95% and 17.86% (0.125 µg/mL) in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed a notable cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 15.68%, 36.47%, and 29.64%, respectively at 2.5 µg/mL compared with the untreated group. The significant wound healing activity was reported in scratch assay in HFF-1 and HaCaT cells due to the significantly higher cellular migration of fibroblast and keratinocytes. Therefore, Biofield Energy Treatment (The Trivedi Effect®) based test formulation and cell medium might be a suitable approach for the development for herbal cosmetics and formulations as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action. It can improve the overall skin health care against many skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, etc.

The study results conclude that The Trivedi Effect®-Consciousness Energy Healing Treatment based test formulation and cell medium (DMEM), have the potential applications in various skin disorders. MTT assay showed the Biofield Energy Treated test formulation was found to be safe and nontoxic in all the tested concentrations. Fibroblast proliferation assay using BrdU in HFF-1 cells displayed significant proliferation by 91.51% and 201.14% in the UT-DMEM + BT-Test Formulation and BT-DMEM + UT-Test Formulation groups, respectively at 35 µg/mL, compared with the untreated group. Collagen synthesis was significantly increased by 64.58% and 57.38% in the UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation group respectively. However, in BT-DMEM + UT-Test Formulation group showed an increased collagen amount by 38.56%. Besides, the level of elastin was found to be increased by 21.96% and 11.42% in the UT-DMEM + BT-Test Formulation group at concentration 10 and 5 µg/mL respectively, compared to the UT-DMEM + UT-Test Formulation group. Hyaluronic acid concentration was increased by 14.02% (1.25 µg/mL) and 6.57% (0.625 µg/mL) in the BT-DMEM + BT-Test Formulation group compared with the UT-DMEM + UT-Test Formulation. The melanin synthesis inhibition was found in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation group by 12.95% and 17.86% (0.125 µg/mL), respectively compared with the UT-DMEM + UT-Test Formulation group in B16-F10 melanoma cell line. Biofield Energy Treated test formulation showed a significant anti-wrinkling effect using UV-B induced stress in the HFF-1 cells by 15.68%, 36.47%, and 29.64% increase cell viability in the UT-DMEM + BT-Test Formulation, BT-DMEM + UT-Test Formulation, and BT-DMEM + BT-Test Formulation groups, respectively compared with the UT-DMEM + UT-Test Formulation group. Wound healing scratch assay exhibited significant improved wound closure area up to 9% after treatment with the Biofield Energy based test formulation with increased cellular migration of fibroblast and keratinocytes in the HFF-1 and HaCaT cells. Thus, the Biofield Energy Treated Test Formulation and DMEM media can be used to improve various skin disorders via improved collagen, elastin, hyaluronic acid synthesis that helps to improve the skin elasticity and tightness.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, seborrheic dermatitis, chickenpox, measles, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, wrinkles, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.