Skin Nourishing Effects of Biofield Energy Healing Treatment Based Herbomineral Formulation

The aim of the present study was undertaken to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation on skin health. The test formulation and DMEM were separated into two parts. One part of each received the Consciousness Energy Healing Treatment by Shirley Theresa Holmlund and were termed as the Biofield Energy Treated samples, while the other parts were considered as the untreated test samples. MTT assay showed more than 70% cells were viable in all the tested concentrations in three cell lines and that the test formulation was safe and nontoxic. The percent cell proliferation using BrdU assay was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. The collagen synthesis was significantly increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Elastin level was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the untreated group. The level of hyaluronic acid was significantly increased by 15.13% and 68.56% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. The level of melanin was significantly reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL compared to untreated group. The effect of skin protection upon exposure with UV-B rays displayed some alteration of the percent cell viability in all the tested groups. The wound healing activity by scratch assay exhibited significant wound closure and cell migration in the test formulation and DMEM in HFF-1 cells compared to the untreated group. Altogether, the data suggests that the Consciousness Energy Healing Treated DMEM and test formulation exhibited better skin protective responses compared to the untreated DMEM and test formulation group with respect to the skin health parameters. Therefore, the Biofield Energy Treated test formulation could be developed as a potential cosmetic product against various skin problems viz. eczema, vitiligo, psoriasis, acne, hives, rosacea, ichthyosis, seborrheic dermatitis, erythema, contact dermatitis, skin aging, wrinkles, etc.

The cell viability by MTT assay exhibited more than 70% cells were viable in all the tested concentrations. The percent cell proliferation using BrdU assay at 8.75 µg/mL was significantly increased by 343.57%, 100.29%, and 132.6% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. The level of collagen was increased by 5.94% and 10.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of elastin was significantly (p≤0.001) increased by 15.95%, 58.31%, and 47.18% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Moreover, elastin level at 10 µg/mL was significantly (p≤0.001) increased by 119.73%, 76.98%, and 181.73% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was increased by 15.13% and 68.56% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. However, it was also increased by 5.18% and 56.39% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the UT-DMEM + UT-Test formulation group. Melanin level was reduced by 17.06%, 6.94%, and 13.07% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.063 µg/mL with respect to the UT-DMEM +UT-Test formulation group. Protection with respect to UV-B, showed that the percent cell viability was altered minimally in the different test concentrations. Wound healing results showed significant effects on wound closure and cell migration in the test formulation and DMEM groups in HFF-1 cells compared to the untreated group. Overall, the Biofield Energy Treated test formulation (The Trivedi Effect®) and DMEM have shown significant protective effects on various skin health parameters such as skin whitening, aging, wrinkling, and wound healing. Therefore, the Biofield Energy Healing based test formulation could be suitable for the development of herbal cosmetics, and it would be useful for the management of wounds and various skin related disorders viz. cellulitis, abscess, syringoma, pimple, impetigo, scabies, photosensitivity, urticaria, hives, warts, acne, abscess, callus, skin aging, wrinkles and/or change in skin color, chickenpox, eczema, rosacea, seborrheic dermatitis, athlete's foot, psoriasis, contact dermatitis, cutis rhomboidalis nuchae, erythema, etc.