Skin Photo-Protective and Anti-ageing Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation

The objective of this study was to evaluate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment based herbomineral formulation on various skin parameters using HFF-1, HaCaT, and B16-F1 cell lines. The test formulation was composed of minerals (zinc, selenium, and molybdenum), and L-ascorbic acid along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). Test formulation and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment by Bonnie P. Hegarty-Diaz and denoted as treated, while other part was coded as untreated groups. MTT assay result showed that the test formulation was found safe and nontoxic at tested concentration 40 µg/mL. Fibroblast cell proliferation assay showed significant increased cell proliferation by 26.83% at 8.75 µg/mL in BT-DMEM + BT-Test formulation group compared to the untreated group. The level of collagen was significantly increased by 22.05% and 22.24% (at 2.5 µg/mL) in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively. However, collagen amount was increased by 11.08% and 9.52% in BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL, compared to untreated group. Similarly, elastin synthesis was increased in the BT-DMEM + UT-Test formulation group by 19.98%, 72.54% and 105.04% at the concentrations 10, 5, and 2.5 µg/mL, respectively compared to the untreated group. However, level of hyaluronic acid was increased by 5.19% at 0.625 µg/mL in UT-DMEM + BT-Test formulation group compared with the untreated group. Besides, melanin synthesis inhibition was found to be inhibited by 14.80% and 17.40% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling activity in HFF-1 cells showed improve cell viability in all the groups at various concentrations, i.e. in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation group by 10.69%, 6.9%3, and 11.86%, respectively at 0.625 µg/mL compared with the untreated group. In addition, wound healing scratch assay data suggest significantly higher cellular migration of fibroblast and keratinocytes cells in HFF-1 and HaCaT cells lines, respectively. In conclusion, Biofield Energy Healing (The Trivedi Effect®) based test formulation and DMEM could be beneficial against various skin disorders such psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections and can be significantly used as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action.

Present experimental results showed significant impact of the Biofield Energy Healing based herbomineral test formulation and DMEM against various skin parameters. Cell viability using MTT assay showed that the Biofield Energy Healing based herbomineral test formulation was safe and nontoxic in all the tested concentrations. Fibroblast proliferation assay using BrdU assay in HFF-1 cells exhibited significant proliferation increased by 26.83% at 8.75 µg/mL in BT-Test formulation + BT-DMEM group compared with the untreated group. Collagen synthesis was improved by 22.05% and 22.24% at 2.5 µg/mL in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with untreated group. Besides, the level of elastin was found to be increased by 29.41% and 27.55% in BT-DMEM + BT-Test formulation group at 10 and 5 µg/mL respectively, compared to the UT-DMEM + UT-Test formulation group. However, the amount of hyaluronic acid was increased by 5.19% in UT-DMEM + BT-Test formulation group compared with the UT-DMEM + UT-Test formulation at concentration 0.625 µg/mL. The melanin synthesis inhibition was found at 0.125 µg/mL by 14.80% and 17.40% in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the UT-DMEM + UT-Test formulation group in B16-F10 melanoma cell line. However, the Biofield Energy Treated Test formulation showed significantly improved anti-wrinkling effects using UV-B induced stress in HFF-1 cells by 11.7% and 14.99% in UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 2.5 µg/mL, while 10.69%, 6.93%, and 11.86% in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared with the UT-DMEM + UT-Test formulation group. Wound healing activity using scratch assay demonstrated significant migration of fibroblast and keratinocytes cells with increased covered area up to 5% after treatment with the Biofield Energy Healing based herbomineral test formulation in HFF-1 and HaCaT cells. Hence, the Biofield Energy Treated test herbomineral formulation could be a better alternative approach against skin disorders through improved collagen, elastin, and hyaluronic acid synthesis with significant wound healing effect.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing. Further, the Biofield Energy Healing based herbomineral test formulation can also be used in the prevention of temporary and permanent skin disorders, anti-aging, improved overall health, and quality of life.