Journal: Journal of Food and Nutrition Sciences PDF
Published: 9-May-17 Volume: 5 Issue: 3 Pages: 86-95
DOI: 10.11648/j.jfns.20170503.15 ISSN: 2330-7285 (Print) 2330-7293 (Online)
Authors: Dennille Mellesia Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Citation: Dennille Mellesia Smith, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana. Skin Protective Activity of Consciousness Energy Healing Treatment Based Herbomineral Formulation. Journal of Food and Nutrition Sciences. Vol. 5, No. 3, 2017, pp. 86-95. doi: 10.11648/j.jfns.20170503.15
The current study was attempted to evaluate the impact of the Consciousness Energy Healing (The Trivedi Effect®) Treatment based herbomineral test formulation and cell medium (DMEM) against skin health. The test formulation and DMEM were divided into two parts. One of each part was received the Consciousness Energy Healing Treatment by Dennille Mellesia Smith and was termed as the Biofield Energy Treated samples, while the other parts were denoted as the untreated test items. MTT showed >78% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 17.5 µg/mL with respect to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the untreated group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was increased by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. The level of melanin was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation, respectively at 0.13 µg/mL compared to the untreated group. Skin protection against UV-B data displayed that cell proliferation was increased by 17.88%, 20.14%, and 9.89% in the BT-DMEM + BT-Test formulation at 0.63, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Wound healing activity exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, result suggests that the Biofield Energy Treated DMEM and test formulation exhibited better responses compared to the untreated medium and test formulation. Therefore, the Biofield Energy Treated herbomineral formulation could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc.
The cell viability using MTT assay exhibited more than 78% cells were viable, indicating that the Biofield Energy Healing based herbomineral test formulation was safe and nontoxic in all the tested concentrations. The percent cell proliferation using BrdU was significantly increased by 238.30%, 192.06%, and 43.96% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BTDMEM + BT-Test formulation groups, respectively at 17.5 µg/mL compared to the UT-DMEM + UT-Test formulation group. The level of collagen was significantly increased by 55.55%, 32.65%, and 52.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation and BTDMEM + BT-Test formulation groups, respectively at 1.25 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Elastin was significantly (p≤0.001) increased by 6.30%, 105.04%, and 29.41% at 10 µg/mL in the UT-DMEM + BTTest formulation, BT-DMEM + UT-Test formulation, and BTDMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was increased significantly by 4.78%, 29.71%, and 58.29% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test Formulation group. Melanin level was significantly (p≤0.001) reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UTTest formulation groups, respectively at 0.13 µg/mL with respect to the UT-DMEM + UT-Test formulation group. Antiwrinkling effect using UV-B induced stress in HFF-1 cells showed that the Biofield Energy Treated test formulation was increased significantly by 17.88%, 20.14%, and 9.89% at 0.63, 1.25, and 2.5 µg/mL, respectively in the BT-DMEM + BTTest formulation group compared to the UT-DMEM + UTTest formulation group. Wound healing results displayed a significant effect of the test formulation and DMEM on wound closure and cell migration in all the tested groups in both HFF1 and HaCaT cells compared to the untreated groups. Overall, the Consciousness Energy Healing Treated test formulation (The Trivedi Effect®) and DMEM have shown significant protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Therefore, the Biofield Energy Healing based herbomineral test formulation would be suitable for the development of herbal cosmetics, and it would be useful for the management of wounds and various skin related disorders viz. abscess, pimple, cellulitis, impetigo, scabies, syringoma, photosensitivity, urticaria, hives, warts, abscess, callus, acne, chickenpox, eczema, rosacea, seborrheic dermatitis, athlete's foot, psoriasis, erythema, contact dermatitis, cutis rhomboidalis nuchae, skin aging, wrinkles and/or change in skin color etc.