Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation

Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.

MTT cell viability assay data showed more than 70% cells were viable in all the tested concentrations. BrdU assay showed that the percent cell proliferation was significantly increased by 48.06%, 451.42%, and 722.48% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively compared to the UT-DMEM + UT-Test formulation group. Collagen level was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to UT-DMEM + UT-Test formulation group. The level of elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-Test formulation group. Hyaluronic acid was significantly increased by 11.21% and 10.45% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.63 µg/mL compared to the UT-DMEM + UT-Test formulation group. Melanin level was reduced by 12.49% in the UT-DMEM + BT-Test formulation at 0.01 µg/mL with respect to the untreated group UT-DMEM + UT-Test formulation group. Moreover, the level of melanin was also decreased by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the UT-DMEM + UT-Test formulation group. Protection with respect to UV-B, showed that the percent cell viability was increased by 4.23% at 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Wound healing results showed significant effects on wound closure and cell migration in the test formulation and DMEM groups in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the Biofield Energy Treated test formulation (The Trivedi Effect®) has shown significant protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Therefore, the Consciousness Energy Healing Treatment based test formulation could be suitable for the development of herbal skin preparations, which would be very useful for the management of wounds and various skin related disorders such as acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, etc.