The Impact of Biofield Energy Treated Vitamin D3 on the Structural Crosslinks, Maturation, and Bone Mineralization in Human Bone Osteosarcoma Cells (MG-63)

The objective was to investigate the potential of Biofield Treated vitamin D3 and DMEM on bone markers. The test items (TIs) were separated into two parts. One part of each sample was received Consciousness Energy Treatment by Alice Branton and termed as Biofield Treated (BT) samples, while other parts of each sample denoted as untreated TI (UT). MTT data found TIs were safe and nontoxic at tested concentrations. ALP was significantly elevated by 390% and 200% in UT-DMEM + BT-TI at 0.1 and 1µg/mL, respectively; while, increased by 135.29% in BT-DMEM + BT-TI at 0.1µg/mL than UT-DMEM + UT-TI. ALP was significantly increased by 106.34%, 138.47%, and 212.68% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Collagen was significantly increased by 288.68% and 106.96% in UT-DMEM + BT-TI and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Further, collagen was significantly increased by 435.78% and 139.72% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 50µg/mL than untreated. Percent of bone mineralization was significantly increased by 299.13%, 239.91%, and 218.77% in BT-DMEM + BT-TI at 0.1, 1, and 10µg/mL, respectively than untreated. Besides, percent of bone mineralization was distinctly increased by 192.51% in BT-DMEM + UT-TI at 10µg/mL than untreated. Overall, Biofield Treated vitamin D3 remarkably improved bone parameters in MG-63 cells and could be an alternative to vitamin D3 deficiency and used various bone disorders viz. low bone density, osteoporosis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

The MTT cell viability assay data showed more than 81% cells were viable, which indicated that the test samples were safe and nontoxic in all the tested concentrations. ALP level was significantly elevated by 200% and 135.29% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 1µg/mL while, it was also increased by 390% in the UT-DMEM + BT-Test item at 0.1µg/mL compared to the UT-DMEM + UT-Test item group. Further, ALP was significantly increased by 106.34%, 138.47%, and 212.68% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 288.68% and 106.96% in the UT-DMEM + BT-Test item and BT-DMEM + B T-Test item groups, respectively at 10µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 435.78%, 139.72%, and 70.63% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 50µg/mL compared to the untreated group. The percent of bone mineralization was significantly enhanced by 125.66% and 299.13% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 0.1µg/mL however, it was also increased by 144.17% and 239.91% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 1µg/mL compared to the UT-DMEM + UT-Test item group. Besides, the percent of bone mineralization was distinctly increased by 192.51% and 218.77% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 10µg/mL compared to the untreated group. A noticeably increased the percent of bone mineralization by 163.97% and 183.39% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 50µg/mL with respect to the untreated group. Altogether, the Biofield Energy Treated test samples (The Trivedi Effect®) showed a significant impact on bone health parameters.

Therefore, the Consciousness Energy Healing-based vitamin D3 might be suitable as supplement for vitamin D3 deficiency, which could be useful for the management of various bone-related disorders viz. low bone density, osteomalacia, osteoporosis, rickets, osteogenesis imperfecta, osteoma, Paget’s disease, deformed bones, chondrodystrophia fetalis, etc. Besides, it can also be used in organ transplants (kidney, liver, and heart transplants), autoimmune disorders (Addison Disease, Celiac Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Systemic Lupus Erythematosus, Myasthenia Gravis, Type 1 Diabetes, Pernicious Anemia, Aplastic Anemia, Rheumatoid Arthritis, Alopecia Areata, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders (Ulcerative Colitis, Crohn’s Disease, Irritable Bowel Syndrome). Further, it could be beneficial for the management of Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, and Hepatitis, anti-stress, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic actions and anti-aging by improving overall health.