The Study of Biofield Energy Treatment Based Herbomineral Formulation in Skin Health and Function

The aim of the study was to evaluate the effect of the The Trivedi Effect®- Consciousness Energy Healing Treatment based new proprietary herbomineral formulation and cell medium (DMEM) on various skin health parameters using HFF-1, HaCaT, and B16-F10 cell lines. It contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, along with the mixture of Centella asiatica extract and tetrahydrocurcumin (THC). The test formulation and DMEM were divided into two parts each, one part of each was coded as untreated (UT), while the second part of each was treated with the Biofield Treatment by Johanne Dodon and denoted as the Biofield Energy Treated (BT) test items. MTT assay result showed that the test formulation was safe and nontoxic up to 80 µg/mL concentration. The fibroblast cell proliferation assay using BrdU method showed a significant increased cell proliferation at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54%, respectively in the BT-DMEM + BT-Test formulation group compared to the untreated group. The collagen data showed an increased synthesis by 7.42% and 3.84% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL with respect to the untreated group. Similarly, the elastin synthesis was increased by 19.8% and 25.8% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL with respect to the untreated group. However, the Biofield Energy Treated test formulation showed an increased level of hyaluronic acid by 1.7% in UT-DMEM + BT-Test formulation group compared to the untreated group. Melanin synthesis inhibition was observed by 15.21% and 11.43% in UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation groups, respectively at 0.125 µg/mL compared with the untreated group in B16-F10 melanoma cell line. Anti-wrinkling effects showed an improved cell viability by 7.65% at 1.25 µg/mL in the BT-DMEM + BT-Test formulation experimental group compared with the untreated test group. In addition, the Biofield Energy Treated Test formulation/DMEM showed a significant wound healing activity and high cellular migration of fibroblast and keratinocytes in HFF-1 and HaCaT cells, respectively. Overall, the data suggested that Biofield Treated formulation/DMEM could be helpful for skin healing, improvement the skin health, elasticity, and tightness, as well as, improving skin health issues such as wrinkling, pigmentation disorders, redness, swelling, skin cancer, aging, rashes, and skin sagging.

The current study established the potential application of the Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing Treatment) based test formulation and cell medium with respect to the different skin health parameters using human fibroblasts, human keratinocytes, and mouse melanoma cell lines (i.e. HFF-1, HaCaT, and B16-F10). MTT assay results showed that the test formulation was found to be safe up to 80 µg/mL concentration. Fibroblast proliferation assay in HFF-1 cells showed a significant proliferation rate at 2.13, 8.75, and 17.53 µg/mL by 3.90%, 14.68% and 29.54% in the BT-DMEM + BT-Test formulation group compared to the untreated group. Among tested ECM components, 3.84% to 7.42% increases in collagen were reported compared with the untreated test formulation and DMEM. Similarly, the elastin level was increased by 19.8% and 25.8% in UT-DMEM + BT-Test Formulation (5 µg/mL; p≤0.05) and BT-DMEM + BT-Test Formulation (5 µg/mL; p≤0.01) groups, respectively compared with the UT-Test Formulation and UT-DMEM. In addition, the level of hyaluronic acid was increased by 1.7% in UT-DMEM + BT-Test Formulation group compared with untreated test formulation and DMEM. Besides, melanin synthesis inhibition was found to be in range of 5.79% to 15.21% in different groups, while UT-DMEM + BT-Test Formulation and BT-DMEM + BT-Test Formulation group showed a maximum inhibition of melanin synthesis by 15.21% and 11.43%, respectively at 0.125 µg/mL compared with the untreated Test Formulation and DMEM in B16-F10 melanoma cell line. An anti-wrinkling effects using UV-B induced stress in HFF-1 cells of the Biofield Treated test formulation showed 7.65% improved cell viability in the BT-DMEM + BT-Test Formulation experimental group at 1.25 µg/mL compared with the UT-DMEM + UT-Test formulation group. The wound-healing scratch assay showed a significantly increased cellular proliferation and migration in HFF-1 and HaCaT cells in the Biofield Energy Treated Test Formulation compared with the untreated test formulation and DMEM. Overall, the Biofield Energy Healing (The Trivedi Effect®) based test formulation has shown substantial protective effects on skin disorders such as wound healing, wrinkling, ageing and hyperpigmentation. Therefore, the Biofield Energy based novel herbal pharmaceuticals might be a suitable approach for the development for herbal cosmetics and their formulation, which can improve the overall skin health care with respect to anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating action.

Overall, the Biofield Energy Treated test formulation can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various skin irregularities that are typically symptoms of a skin disorders such as Eczema, warts, acne, hives, ringworm, Rosacea, psoriasis, skin cancer, wrinkles, diaper rash, seborrheic dermatitis, chickenpox, measles, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, excessive flushing. Further, the Biofield Energy Healing Treatment can also be used in the prevention of temporary and permanent skin disorders, anti-aging, and in the improvement of overall health and quality of life.