Vitamin D3 and Consciousness Energy Healing Treatment Modality on Human Osteoblast Cells

The current study was established to evaluate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone cells development. The test items were divided into two parts. One part of each sample received Consciousness Energy Healing Treatment by Jay Antony Prague and those samples were labeled as Biofield Treated (BT) samples, while other parts of each sample were denoted as untreated test items (UT). MTT data showed test samples were found as safe in tested concentrations. ALP was significantly increased by 214.74%, 350.53%, and 536.32% in UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to UT-DMEM + UT-Test item group. Further, ALP level was significantly elevated by 155.09% and 93.37% in BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 1 µg/mL compared to untreated. Collagen was significantly increased by 73.40%, 150.39%, and 133.59% in UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 1 µg/mL compared to untreated. Moreover, level of collagen was significantly increased by 105.68%, and 72.76% in BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to untreated. Besides, percent of bone mineralization was distinctly increased by 229.99%, 229.99%, and 200% in UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1µg/mL compared to untreated. Percent of bone mineralization was distinctly increased by 137.27% and 142.17% in UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 100 µg/mL, while increased by 78.29% and 96.71% in UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively as compared to untreated. Overall, Biofield energized vitamin D3 has a significant effect on bone health and able to combat vitamin D3 deficiency and fight against various bone-related problems.

For the assessment of cytotoxic nature of the test items, the MTT cell viability assay was performed and the data showed greater than 70% cells were viable, which indicated that the test samples were safe and nontoxic in the tested concentrations upto 100 µg/mL. ALP was significantly increased by 214.74%, 350.53%, and 536.32% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL; while increased by 155.09% and 93.37% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item group at 1 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 73.40%, 150.39%, and 133.59% UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 1µg/mL with respect to the untreated group. Additionally, the level of collagen was significantly increased by 105.68%, and 72.76% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL with respect to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 229.99%, 229.99%, and 200% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 137.27% and 142.17% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 100 µg/mL, while increased by 78.29% and 96.71% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively as compared to the untreated group. Altogether, the Biofield Energy Treated test samples (The Trivedi Effect®) demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing based vitamin D3 might be suitable for the development of an alternative and more effective supplement for vitamin D3 deficiency, which could be useful for the management of various bone-related disorders viz. low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and joint pain, bone fractures, deformed bones, osteoma, chondrodystrophia fetalis, etc. Besides, it can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Reactive Arthritis, Rheumatoid Arthritis, Sjogren Syndrome, Systemic Lupus Erythematosus, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as inflammatory disorders such as Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome, inflammatory diseases, anti-inflammatory, anti-stress, anti-arthritic, anti-osteoporosis, anti-apoptotic, wound healing, anti-cancer, anti-psychotic and anti-fibrotic actions stress management and prevention, and anti-aging by improving overall health, Parkinson’s Disease and stress etc. To modulate the immune system by improving overall health.