Analysis of Genetic Diversity Using Simple Sequence Repeat (SSR) Markers and Growth Regulator Response in Biofield Treated Cotton (Gossypium hirsutum L.)

Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi biofield energy and referred as treated sample. The growth-germination of cotton seedling data showed higher germination (82%) in biofield treated seeds as compared to the control (68%). The alterations in length of shoot and root of cotton seedling was reported in the treated sample with respect to untreated seeds. However, the endogenous level of GSH in the leaves of treated cotton was increased by 27.68% as compared to the untreated sample, which may suggest an improved immunity of cotton plant. Further, the plant growth regulatory constituent i.e. IAA concentration was increased by 7.39%, as compared with the control. Besides, the DNA fingerprinting data, showed polymorphism (4%) between treated and untreated samples of cotton. The overall results suggest that the biofield energy treatment on cotton seeds, results in improved overall growth of plant, increase germination rate, GSH and IAA concentration were increased. The study assumed that biofield energy treatment on cotton seeds would be more useful for the production of cotton fiber.

In conclusion, biofield energy treated cotton seeds resulted in enhanced germination rate by 20% as compared with the control. Further, the length of the shoot and root of cotton plant after biofield energy treatment was reported to be increased as compared with the control. Moreover, GSH level (i.e. a biochemical marker for immunity level) in plant cell of biofield treated cotton seeds was increased by 27.68%, which suggest increased immunity of cotton plants after biofield treatment. The IAA concentration after biofield treatment was increased by 7.39%, which may inhibit the growth of phytopathogens, and hence improved the overall growth of the plant. Polymorphism was detected between treated and untreated samples of cotton seeds. The percentage of polymorphism observed between treated and untreated samples was 4%; which could be a notable data in support of biofield treatment on cotton. Based on study outcome, Mr. Trivedi’s biofield energy could be used as better alternate approach to improve the overall yield of agricultural crops in near future.