Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone

Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.

The XRD study revealed the amorphous nature of bio peptone in both the control and treated samples. The particle size analysis showed the increase in the particle sizes i.e. d50 and d99 of the treated bio peptone as compared to the control. The surface area analysis showed the increase in the effective surface area of treated sample by 253.95% as compared to the control. The DSC study showed the significant increase in the melting temperature of treated sample by 29.59% as compared to the control. Furthermore, the TGA/DTG study revealed the slight increase in onset temperature of thermal degradation from 181°C (control) to 187°C in treated sample. This revealed that thermal stability of the treated sample was increased as compared to the control. The FT-IR study revealed the alteration in wavenumber corresponding to N-H, C-H, C=C, C-O, and P=O vibrations after biofield energy treatment as compared to the control sample.

Based on these results, it is concluded that Mr. Trivedi’s biofield energy treatment had the considerable impact on the various physicochemical and spectroscopic properties of bio peptone. Further, it is expected that the biofield energy treated bio peptone could serve as a better component of culture media with respect to the thermal stability.