Determination of Isotopic Abundance Ratio of Biofield Energy Treated 1,4-Dichlorobenzene Using Gas Chromatography-Mass Spectrometry (GC-MS)

The objective of the current study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of PM+1/PM, PM+2/PM, PM+3/PM and PM+4/PM in p-DCB using gas chromatography-mass spectrometry (GC-MS). The p-DCB was divided into two parts – one part was control sample, and another part was considered as the treated sample which was subjected to biofield energy treatment (The Trivedi Effect®). T1, T2, T3, and T4 were referred the biofield treated p-DCB having analyzed at different time intervals. The GC-MS analysis of both the control and biofield treated p-DCB indicated the presence of the parent molecular ion peak at m/z 146 along with four major fragmentation peaks at m/z 111, 75, 55 and 50. The relative peak intensities of the fragmented ions in the biofield treated p-DCB were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of PM+1/PM at T1, T2, T3, and T4 (biofield energy treated p-DCB) was significantly increased by 10.87, 83.90, 225.16, and 241.15%, respectively as compared to the control sample. Consequently, the percentage change in the isotopic abundance ratio of PM+2/PM at T1, T2, and T3 (biofield energy treated p-DCB) was enhanced by 4.55, 9.49, and 1.80%, respectively as compared to the control sample. Beside these, another two isotopic molecular ion peaks at m/z 149 and 150 were found in the GS-MS spectra due to arise from the contributions of various combinations of 2H, 13C, and 37Cl. The isotopic abundance ratios of PM+3/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 15.14, 82.57, 192.43, and 218.31%, respectively as compared to the control sample. Similarly, the PM+4/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 13.80, 86.66, 186.13, and 204.29%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM(2H/1H or 13C/12C), PM+2/PM(37Cl/35Cl), for PM+3/PM and PM+4/PM (the probable combinations of 2H/1H, 13C/12C, and 37Cl/35Cl) were significantly enhanced in the biofield energy treated p-DCB. The biofield treated p-DCB has shown improved isotopic abundance ratios that might have altered the physicochemical properties, thermal properties and rate of reaction. Biofield treated p-DCB might be useful in pharmaceutical and chemical industries as intermediates during the manufacturing of pharmaceuticals and chemicals by monitoring the rate of chemical reaction.

The current study inferred that biofield energy treatment has a remarkable capability for altering the isotopic abundance ratio in p-DCB. The gas chromatography-mass spectrometric (GC-MS) analysis of the both control and biofield energy treated sample showed the presence of the molecular ion peak at m/z 146 (calculated 145.97 for C6H4Cl2+) along with four major fragmented peaks at m/z 111, 75, 55 and 50. Only, the relative peak intensities of the fragmented ions in treated sample were different from the control sample. The isotopic abundance ratios analysis in p-DCB revealed that the isotopic abundance ratio of PM+1/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 10.87, 83.90, 225.16, and 241.15%, respectively as compared to the control. Consequently, the isotopic abundance ratio of PM+2/PM was enhanced in biofield energy treated sample at T1, T2, and T3 by 4.55, 9.49, and 1.80%, respectively as compared to the control. Subsequently, the PM+3/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 15.14, 82.57, 192.43, and 218.31%, respectively as compared to the control sample. Similarly, the isotopic abundance ratio of PM+4/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly improved by 13.80, 86.66, 186.13, and 204.29%, respectively as compared to the control sample. It was observed that the isotopic abundance ratios of PM+1/PM, PM+3/PM, and PM+4/PM in the biofield treated sample were increased gradually with respect to time. As biofield energy treated p-DCB had increased isotopic abundance ratio, it might have altered physicochemical and thermal properties, and rate of reaction than the control sample. Hence, biofield energy treated p-DCB could be advantageous in pharmaceutical and chemical industries as intermediates during the preparation of pharmaceuticals and chemicals by controlling the rate of chemical reaction.