Evaluation of the Consciousness Energy Healing Treated Berberine Chloride Using PXRD, PSA, and DSC Analysis

Berberine is one of the well-known traditional Chinese medicines and it has been significantly effective against various ailments. This study was performed to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on its physicochemical properties by using PXRD, PSA, TGA/DTG, and DSC analytical techniques. The sample was divided into two parts; the first part was considered as a control sample (without treatment), while the second part was received the Consciousness Energy Healing Treatment remotely by the Biofield Energy Healer, Gopal Nayak and denoted as the treated sample. The PXRD peak intensities and crystallite sizes were altered ranging from -28.78% to 115.15% and - 15.76% to 241.38%, respectively; however the average crystallite size was increased significantly by 23.05% in the treated sample compared to the control sample. The particle sizes were significantly increased by 16.89% (d10), 34.41% (d50), 21.88% (d90), and 22.85 % {D (4,3)}, which resulted in the significant increase in the specific surface area by 14.74% in the treated sample compared to the control sample. The thermal analysis indicated 3.98% increase in weight loss and 3.64% decrease in the residue weight of the treated sample compared to the control sample. The latent heat for the 1st, 2nd, 3rd, 4th, 5th, and 6th peaks of the treated sample were significantly altered by 224.78%, 7.93%, 8.92%, - 9.05%, -45.82%, and 10.93%, respectively, compared to the control sample. The overall analysis indicated that the Trivedi Effect®-Consciousness Energy Healing Treated berberine chloride might possess better solubility, absorption, and thermal stability compared to the untreated sample. Thus, the Trivedi Effect® could be used in improving the bioavailability of berberine chloride and might be used for designing the novel nutraceutical/pharmaceutical formulations to combat various diseases.

The Trivedi Effect®-Consciousness Energy Healing Treatment has shown a significant impact on the physicochemical properties of berberine chloride in comparison to the control sample. The PXRD peak intensities and crystallite sizes altered ranging from28.78% to 115.15% and -15.76% to 241.38%, respectively, in comparison to the control sample. Besides, the average crystallite size of the treated sample was significantly increased by 23.05% compared to the untreated berberine chloride sample. It is suggested that such changes have taken place as the Biofield Energy Treatment might create some new polymorph of berberine chloride that could be evident from the major changes in the crystalline properties of the treated sample. The particle size data showed significant variations in the size distribution of the berberine sample after the Biofield Energy Treatment as the particle sizes were significantly reduced by 16.89%, 34.41%, 21.88%, and 22.85%, at d10, d50, d90, and D(4,3), respectively in comparison to the control sample. The changes in the particle sizes affected thespecific surface area of the treated sample, which was observed to be increased by 14.74% compared to the control sample. Such changes might attribute the significant alterations in the bioavailability profile of the treated sample by enhancing the solubility, dissolution, and absorption parameters. The TGA study revealed the increase i.e., 3.98%, in the total weight loss of the treated sample and 3.64% decrease in the residue weight of the treated berberine chloride compared to the untreated sample. However, the Tmax corresponding to the 1st, 2nd, 3rd, and 4th peak of the treated sample was increased by 7.04%, 1.73%, 1.38%, and 2.97%, respectively compared to the untreated berberine chloride sample. The DSC data revealed that the melting point of 1st, 2nd, 3rd, and 4th peak of the treated berberine chloride sample were increased by 11.03%, 1.19%, 14.96%, and 2.18%, respectively, compared to the control sample. The latent heat for the 1st, 2nd, 3rd, 4th, 5th, and 6th peaks of the treated sample were significantly altered by 224.78%, 7.93%, 8.92%, - 9.05%, -45.82%, and 10.93%, respectively, compared to the control sample.The results indicated that the Biofield Energy Treatment might improve the degradation temperature of the treated sample that ultimately helps in improving its thermal stability as compared to the control sample. Thus, it was concluded that the Trivedi Effect®- Consciousness Energy Healing Treatment significantly affect the crystalline properties, particle sizes, surface area, and thermal stability of the berberine chloride sample. The Biofield Energy Treatment might form a new polymorph that may show better solubility, absorption,thermal stability, and bioavailability in comparison to the untreated sample. Hence, the Biofield EnergyTreated sample could be used in the form ofnovel drug and might be used to formulate new pharmaceutical/nutraceutical products that would be more effective in the treatment of various diseases such as glaucoma, ailments related to eye and skin, diarrhea, gastric ulcers, tumor, polycystic ovary syndrome, gastroenteritis, menopausal symptoms, hypertension, high cholesterol, inflammation, malaria, hyperglycemia, congestive heart failure, arrhythmia, metabolic syndromes, thrombocytopenia, obesity, osteoporosis, liver diseases, trachoma, etc.