Isotopic Abundance Analysis of Biofield Treated Benzene, Toluene and p-Xylene Using Gas Chromatography-Mass Spectrometry (GC-MS)

Benzene, toluene and p-xylene are derivatives of benzene, generally produced from crude petroleum and have numerous applications in industry. The aim of the present study was to evaluate the impact of biofield treatment on isotopic abundance of these benzene derivatives by gas chromatography-mass spectrometry (GC-MS). Benzene, toluene and p-xylene samples were divided into two parts: control and treatment. Control part was remained as untreated and treatment part was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using GC-MS. GC-MS data revealed that isotopic abundance ratio of 13C/12C or 2H/1H (PM+1/PM) of treated samples were significantly increased from un-substituted to substituted benzene rings (where, PM- primary molecule, PM+1- isotopic molecule either for 13C/12C and/or 2H/1H). The isotopic abundance ratio of 13C/12C or 2H/1H (PM+1/PM) in benzene was decreased significantly by 42.14% as compared to control. However, the isotopic abundance ratio of (PM+1/PM) in treated toluene and p-xylene was significantly increased up to 531.61% and 134.34% respectively as compared to their respective control. Thus, overall data suggest that biofield treatment has significantly altered the isotopic abundance ratio of (PM+1/PM) in a different way for un-substituted and substituted benzenes.

In summary, the biofield treatment has significantly changed the isotopic ratio of (PM+1/PM) in benzene, toluene and p-xylene. Benzene molecule with lower isotopic ratio of (PM+1/PM), might have lower stability due to the decreased µ (reduced mass) and binding energy in molecules with lighter isotopes. In case of toluene and p-xylene, the isotopic abundance ratio of (PM+1/PM) treated samples increased significantly as compared to control. The increased isotopic abundance may increase the binding energy of the chemical bonds in toluene and p-xylene, with increased µ (reduced mass). The observed fragmentation pattern and number of fragmented peaks in mass spectra were same for control and treated toluene and p-xylene samples. These nuclear level transformations of 12C?13C or 1H?2H, were observed which probably induced through biofield treatment. Benzene was less frequently degraded bio-chemically than substituted aromatics i.e., toluene and p-xylene. The decreased isotopic ratio of (PM+1/PM) of benzene after biofield treatment might reduce its stability which could be helpful for biodegradation of benzene in bioremediation of benzene contaminated aquifers. On the contrary increased isotopic ratio of (PM+1/PM) in toluene and p-xylene may increase the stability of toluene and p-xylene may be useful as ingredient of gasoline.