Mass Spectrometry Analysis of Isotopic Abundance of 13C, 2H, or 15N in Biofield Energy Treated Aminopyridine Derivatives

2-Aminopyridine (2-AP) and 2,6-diaminopyridine (2,6-DAP) are two derivatives of aminopyridines that act as an important organic intermediates, mostly used in medicines, dyes and organic sensors. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, or 15N/14N, in aminopyridine derivatives using gas chromatography-mass spectrometry (GC-MS). The 2-AP and 2,6-DAP samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. The GC-MS spectra of 2-AP and 2,6-DAP showed five and six m/z peaks respectively due to the molecular ion peak and fragmented peaks of aminopyridine derivatives. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N were calculated for both the derivatives and significant alteration was found in the treated samples as compared to the respective control. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N in treated samples of 2-AP was decreased by 55.83% in T1 and significantly increased by 202.26% in T4. However, in case of 2,6-DAP, the isotopic abundance ratio of 2H/1H, 13C/12C, and 15N/14N, in the treated sample showed a significant increase (up to 370.54% in T3) with respect to the control. GC-MS data suggested that the biofield energy treatment on aminopyridine derivatives had significantly altered the isotopic abundance of 2H, 13C, or 15N in the treated 2-AP and 2,6-DAP as compared to the control.

In summary, aminopyridine derivatives, 2-AP and 2,6-DAP were studied with GC-MS under the influence of biofield energy treatment and observed a significant change in isotope abundance of 2H/1H, 13C/12C or 15N/14N as compared to the respective control samples. The percent change in isotope abundance ratio of (PM+1)/PM was increased upto 202.26% in 2-AP (T4), while the isotopic abundance ratio was increased significantly upto 370.54 in treated 2,6-DAP (T3) sample. The changes in isotopic abundance ratios have significant impact on bond energies and chemical reactivity of the molecules. Due to the enhancement in the isotopic abundance ratio of (PM+1)/PM in 2-AP and 2,6-DAP, the reactivity may be reduced significantly by increase in the effective mass (µ) and the binding energy of the treated sample. It can be concluded from the above observations that the enhancement of heavier isotopes in the aromatic ring as well as the functional groups may decrease the reactivity of the aromatic ring and the functional groups of aminopyridine derivatives, consequently enabling their utility as pharmacophore in the pharmaceutical industry and as an active material in fluorescence sensors.