Structural and Natural Isotopic Abundance Analysis of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect®) Using LC-MS and NMR

The current research article was aimed to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The liquid chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the Trivedi Effect® Treated sample displayed these peaks at Rt of 1.79 and 2.03 min. The ESI-MS spectra of the control and the Trivedi Effect® Treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered as compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two magnesium gluconate ion forms at m/z 447 and 415 in treated sample was significantly decreased by 59.82% and 55.44%, respectively compared with the control sample. The percentage change in the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably decreased in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% compared with the control sample. Consequently, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Thus, the Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergies, aging, inflammatory diseases, immunological disorders, and other chronic infections. The treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors by using its kinetic isotope effects.

LC-ESI-MS analysis indicated that magnesium gluconate in the solution might be existed in situ of three forms, magnesium gluconate, gluconic acid and a chelate form of magnesium gluconate with gluconic acid by coordinate covalent bond. The ESI-MS spectra exposed the presence of the mass for the magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 in positive ionization mode and the mass for the gluconate ion at m/z 195 in negative ionization mode. The fragmentation pattern of the Trivedi Effect® Treated magnesium gluconate was found to be different from the control sample. Consequently, the LC-ESI-MS based isotopic abundance ratio analysis revealed that The Trivedi Effect® has the tremendous impact on the isotopic abundance ratios of PM+1/PM and PM+2/PM in the magnesium gluconate ion. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two forms of magnesium gluconate ion at m/z 447 and 415 in the treated sample was significantly decreased by 59.82% and 55.44%, respectively with respect to the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably diminished in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% as compared to the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C13H27MgO15)+ to m/z 448 as well as from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C13H27MgO15)+ to m/z 449 in the treated sample were significantly altered with respect to the control sample. 13C, 2H, and 17O contributions from (C6H11O7)+ to m/z 196 was enhanced in the treated sample in comparison with the control sample. The treated sample might exhibit isotope effects such as altered physicochemical and thermal properties, rate of the reaction, selectivity and binding energy due to its changed isotopic abundance ratios of PM+1/PM and PM+2/PM as compared to the control sample. The treated magnesium gluconate might be helpful to understand the enzymatic reactions as well as design the novel potent enzyme inhibitors by using its kinetic isotope effects. Besides, The Trivedi Effect® - Consciousness Energy Healing Treatment could be a useful approach in the design of better nutraceutical and/or pharmaceutical formulations that can offer significant therapeutic responses against various diseases such as diabetes mellitus, allergies and septic shock, stress-related disorders like sleep disorder, insomnia, anxiety, depression, Attention Deficit Disorder (ADD), Attention Deficit Hyperactive Disorder (ADHD), mental restlessness (mind chattering), brain frog, low libido, impotency, lack of motivation, mood swings, fear of the future, confusion, migraines, headaches, forgetfulness, overwhelm, loneliness, worthlessness, indecisiveness, frustration, irritability, chronic fatigue, obsessive/compulsive behavior and panic attacks, inflammatory diseases and immunological disorders like Lupus, Systemic Lupus Erythematosus, Hashimoto Thyroiditis, Type 1 Diabetes, Asthma, Chronic peptic ulcers, Tuberculosis, Hepatitis, Chronic active hepatitis, Celiac Disease (gluten-sensitive enteropathy), Addison Disease, Crohn's disease, Graves’ Disease, Pernicious and Aplastic Anemia, Sjogren Syndrome, Irritable Bowel Syndrome (IBS), Multiple Sclerosis, Rheumatoid arthritis, Chronic periodontitis, Ulcerative colitis, Chronic sinusitis, Myasthenia Gravis, Atherosclerosis, Vasculitis, Dermatitis, Diverticulitis, Rheumatoid Arthritis, Reactive Arthritis, Alopecia Areata, Psoriasis, Scleroderma, Fibromyalgia, Chronic Fatigue Syndrome and Vitiligo, aging-related diseases like cardiovascular disease, arthritis, cancer, Alzheimer’s disease, dementia, cataracts, osteoporosis, diabetes, hypertension, glaucoma, hearing loss, Parkinson’s Disease, Huntington’s Disease, Prion Disease, Motor Neurone Disease, Spinocerebellar Ataxia, Spinal muscular atrophy, Amyotrophic lateral sclerosis, Friedreich’s Ataxia, Lewy Body Disease, chronic infections, and much more.